中医药导报2025，Vol.31Issue(6)：31-35,5.DOI:10.13862/j.cn43-1446/r.2025.06.006
小青龙汤对支气管哮喘大鼠嗜酸性粒细胞凋亡机制的调节作用研究
The Regulatory Effect of Xiaoqinglong Decoction(小青龙汤)on the Apoptosis Mechanism of EOS in Rats with Bronchial Asthma
摘要
Abstract
Objective:To investigate the regulatory effect of Xiaoqinglong decoction on the apoptosis mechanism of eosinophils(EOS)in rats with bronchial asthma.Method:Totally 50 SD rats were randomly divided into blank control group,model control group,Xiaoqinglong decoction group,dexamethasone group,and Xiaoqinglong decoction combined with dexamethasone group(combination therapy group),10 rats in each group.Except for the blank control group,after sensitizing rats with(OVA)/Al(OH)3-mixed liquid,asthmatic rat model was replicated by atomizing and stimulating with OVA solution.Each group were administered corresponding drugs via continuous gastric administration for 14 days.Immunofluorescence TUNEL staining was used to detect eosinophil apoptosis in lung tissue,and enzyme-linked immunosorbent assay was employed to measure the levels of transforming growth factor-β(TGF-β),interferon-γ(IFN-γ),interleukin-3(IL-3),and granulocyte-macrophage colony-stimu-lating factor(GM-CSF)in rat serum.Additionally,Western blotting was utilized to assess the expression levels of sialic acid-binding immunoglobulin-like lectin-8(Siglec-8),cysteinyl aspartate specific protease-3(Caspase-3),and eosinophil chemotactic protein(Eotaxin)in lung tissues.Results:The model control group exhibited EOS infiltration and model control group showed lower apoptosis rates in lung tissues than blank control group(P＜0.05).The model control group showed lower Siglec-8 and Caspase-3 expression than blank control group(P<0.05),while higher Eotaxin expression than blank control group(P<0.05).The model control group showed lower serum TGF-β and IFN-γ levels than blank control group(P<0.05),while higher IL-3 and GM-CSF levels than blank control group(P<0.05).The infiltration of EOS in the lung tissue was reduced in the Xiaoqinglong decoction group,dexamethasone group and combination therapy group,and the Xiaoqinglong decoction group,dexamethasone group and combination therapy group showed higher apoptosis rates than model control group(P<0.05).The Xiaoqinglong decoction group,dexamethasone group and combination therapy group showed higher relative expression levels of Siglec-8 and Caspase-3 than model control group(P<0.05),while lower Eotaxin protein expression than model control group(P<0.05).The Xiaoqinglong decoction group,dexamethasone group and combination therapy group showed higher levels of TGF-β and IFN-γ in the serum,than model control group(P<0.05),while lower levels of IL-3 and GM-CSF than model control group(P<0.05).The combination therapy group showed the most significant improvement in rat indicators among all medication groups(Xiaoqin-glong decoction group,dexamethasone group and combination therapy group).Conclusion:Xiaoqinglong decoction may accelerate EOS apoptosis within asthmatic rat's lung tissues through regulating pro-apoptosis factors as well as anti-apoptosis factors.关键词
支气管哮喘/小青龙汤/嗜酸性粒细胞/细胞凋亡/机制/大鼠Key words
bronchial asthma/Xiaoqinglong decoction/eosinophil/apoptosis/mechanism/rat分类
医药卫生引用本文复制引用
南钟浩,孟玲玉,李春雷,舒亮,卢艳,周星宇,包海鹏..小青龙汤对支气管哮喘大鼠嗜酸性粒细胞凋亡机制的调节作用研究[J].中医药导报,2025,31(6):31-35,5.基金项目
内蒙古自治区自然科学基金项目(2021BS08029) （2021BS08029）
内蒙古医学科学院公立医院科研联合基金科技项目(2023GLLH0115) （2023GLLH0115）
内蒙古医科大学科技百万工程联合项目(YKD2020KJBW(LH)070) （YKD2020KJBW(LH）
