中医药导报2025，Vol.31Issue(6)：36-41,6.DOI:10.13862/j.cn43-1446/r.2025.06.007
苍耳温胆汤对变应性鼻炎大鼠TLR4/MyD88/NF-κB信号通路的影响
The Effect of Cang'er Wendan Tang(苍耳温胆汤)on TLR4/MyD88/NF-κB Signaling Pathway in Rats with Allergic Rhinitis
摘要
Abstract
Objective:To investigate the effect of Cang'er Wendan Tang(CEWDT)on the TOLL-like receptor4(TLR4)/myeloid differentiation factor 88(MyD88)/nuclear transcription factor-κB(NF-κB)pathway in allergic rhinitis(AR)rats.Methods:Totall 40 male rats were randomly divided into blank group,model group,cetirizine group and CEWDT group,with 10 rats in each group.Except for the blank group,the rats in the other three groups were sensitised with sensitising solution containing ovalbumin(OVA)and Al(OH)3 to establish an allergic rhinitis rat model.Each group was given corresponding medication intervention for 7 days.The rats'rhinitis symptom scores were observed and recorded.Histological and morphological changes in the nasal mucosa of rats were observed by hematoxylin-eosin(HE)staining.Serum IgE,interleukin-6(IL-6)and IL-10 levels were detected by enzyme-linked immunosorbent assay(ELISA).Nasal mucosal TLR4,MyD88 and NF-κB protein expression levels were detected by protein Western blotting,and TLR4 mRNA,MyD88 mRNA and NF-κB mRNA expression levels were detected by RT-qPCR.Results:After administration,the rats in the model group showed higher rhinitis symptom scores than those in the blank group(P<0.05).The rats in the CEWDT group and the cetirizine group showed lower rhinitis symptom scores than those in the model group(P<0.05).The difference in rhinitis symptom scores between CEWDT group and cetirizine group was not statistical(P＞0.05).HE staining showed that the nasal mucosa tissue of rats in the blank group was structurally intact and normal.The nasal mucosa of rats in the model group was infiltrated by a large number of symptomatic cells and the arrangement of cells was disorganized.The degree of inflammatory cell infiltration and disorganization of cells in the nasal mucosa tissue of rats in the CEWDT group and the cetirizine group was lower than that of the model group.The model group showed higher serum IgE and IL-6 levels than blank group(P<0.05),while lower expression level of IL-10 than blank group(P<0.05).The CEWDT group and cetirizine group showed lower serum IgE and IL-6 levels than model group(P<0.05),while higher level of IL-10 than model group(P<0.05).The CEWDT group showed lower serum IgE and IL-6 levels than cetirizine group(P<0.05),while higher serum IL-10 level than cetirizine group(P<0.05).The model group showed higher relative expression of TLR4,MyD88 and NF-κB pro teins and relative expression of TLR4 mRNA,MyD88 mRNA and NF-κB mRNA than blank group(P<0.05).The CEWDT group and cetirizine group showed lower relative expression of TLR4,MyD88 and NF-κB proteins and relative expression of TLR4 mRNA,MyD88 mRNA and NF-κB mRNA than model group(P<0.05).The CEWDT group showed lower relative expression of TLR4,MyD88 and NF-κB proteins and relative expression of TLR4 mRNA,MyD88 mRNA and NF-κB mRNA than cetirizine group(P<0.05).Conclusion:Cang'er Wendan Tang can improve clinical symptoms and inflammatory responses in AR rats,and its mechanism of action may be related to regulatory of TLR4/MyD88/NF-κB signalling pathway.关键词
变应性鼻炎/苍耳温胆汤/分消走泄法/TLR4/MyD88/NF-κB信号通路/大鼠Key words
allergic rhinitis/Cang'er Wendan Tang/dispersing and discharging method/TOLL-like recep-tor4/myeloid differentiation factor 88/nuclear transcription factor-κB pathway/rat分类
医药卫生引用本文复制引用
李志军,景伟超,王钇杰,李桃丹,常钰昕,王有鹏..苍耳温胆汤对变应性鼻炎大鼠TLR4/MyD88/NF-κB信号通路的影响[J].中医药导报,2025,31(6):36-41,6.基金项目
黑龙江省中医药管理局国医大师学术思想传承(GY2022-24) （GY2022-24）
国家中医药管理局第七批全国老中医药专家学术经验传承工作项目(国中医药人教函[2022]76号) （国中医药人教函[2022]76号）
