中医药导报2025，Vol.31Issue(6)：49-54,66,7.DOI:10.13862/j.cn43-1446/r.2025.06.009
补天大造丸干预早期卵巢功能不全大鼠免疫功能的作用机制
Effects of Butian Dazao Pill(补天大造丸)on Immune Function and Cytokines IL-2,IL-10 and TGF-β in Rats with Premature Ovarian Insufficiency
摘要
Abstract
Objective:To explore the effects of Butian Dazao pill on the immune function and cytokines in rats with premature ovarian insufficiency.Methods:A total of 10 rats were randomly selected as blank group from 60 rats.Except for the blank group,the remaining rats were given a single intraperitoneal injection of cyclophosphamide at a dose of 75 mg/kg to construct a model of ovarian hypofunction.After the successful replication of the model,50 successfully modeled rats were randomly divided into model control group,positive control group,high dose group of Butian Dazao pill(high dose group),medium dose group of Butian Dazao pill(medium dose group),and low dose group of Butian Dazao pill(low dose group),with 10 rats in each group.The high,medium and low dose groups were given Butian Dazao pill at doses of 23.580,11.790,and 5.895 g/kg respectively.The positive control group was given Progynova at a dose of 0.090 mg/(kg·d)for intragastric administration.The model control group and the blank group were given distilled water for intragastric administration.The intragastric administration was started on the day of model construction,once a day for 28 consecutive days.The rats in the model control group were executed during the diestrus stage 2 weeks after model construction,and the rats in the other groups were executed during the diestrus stage after the end of drug administration.The changes in the ovaries,uteri,and tissues were observed by HE staining.The thymus index,spleen index,uterine index,and ovarian index were detected.The expression levels of serum LH,FSH,E2,AMH,INH-B,IL-2,IL-10 and TGF-β were measured.Results:HE staining showed that,compared with the blank group,the ovarian tissues of the rats in the model control group were significantly atrophied under naked-eye observation,and that the number of follicles at all levels was reduced.The follicular structure was damaged in model group,and the corpora lutea were few and poorly developed.Compared with the model control group,the drug intervention groups showed improvements.Sinus follicles,primordial follicles,primary follicles,secondary follicles,and mature follicles could be seen in positive control group,high dose group,medium dose group,and low dose group.The structures of oocytes,cumulus oophorus,zona pellucida,and corona radiata could be seen.The positive control group,high dose group,medium dose group,and low dose group showed more layers of granulosa cells surrounding the follicles than model control group,and they were arranged regularly.The positive control group,high dose group,medium dose group,and low dose group showed more corpora lutea than model control group,and their development was acceptable.Compared with the model control group,the endometrial layer was significantly thickened in positive control group,high dose group,medium dose group,and low dose group showed more.The glandular cavity was enlarged,and the muscular layer was thickened and arranged densely in positive control group,high dose group,medium dose group,and low dose group showed more.Compared with the model control group,the thymus index and spleen index decreased in high,medium and low dose group(P<0.05 or P<0.01).Compared with the model control group,the uterine index and ovarian index increased in high,medium and low dose groups(P<0.01 or P<0.05).Compared with the model control group,the levels of LH and FSH in the serum decreased in high and medium dose group(P<0.01).The level of LH in the serum decreased in low dose group(P<0.05),while the level of FSH showed no significant change in low dose group(P>0.05).Compared with the model control group,the levels of E2,AMH,and INH-B in the serum increased in the high and medium dose groups(P<0.01).The levels of E2 and AMH in the serum increased in low dose group(P<0.05),while the level of INH-B showed no significant change in low dose group(P>0.05).Compared with the model control group,the level of serum IL-2 and TGF-β decreased in Progynova group,high dose group,medium dose group and low dose group(P<0.01 or P<0.05),while the expression of IL-10 increased in Progynova group,high dose group and medium dose group(P<0.01 or P<0.05).Conclusion:Butian Dazao pill can improve the levels of serum reproductive hormones and cytokines in mice with immune premature ovarian insufficiency,reduce the ovarian inflammatory reaction,regulate the immune disorder,improve the ovarian reserve capacity,and play a protective role in the ovaries.关键词
早发性卵巢功能不全/补天大造丸/TGF-β/IL-2/IL-10/大鼠Key words
premature ovarian insufficiency/Butian Dazao pill/TGF-β,IL-2/IL-10/rat分类
医药卫生引用本文复制引用
杨永琴,申剑,王帅哲,马睿玲,胡杨,杨燕..补天大造丸干预早期卵巢功能不全大鼠免疫功能的作用机制[J].中医药导报,2025,31(6):49-54,66,7.基金项目
甘肃省中医药管理局科研课题项目(GZK-2018-10) （GZK-2018-10）
敦煌医学与转化教育部重点实验室开放基金项目(DHYX17-03) （DHYX17-03）
甘肃省高等学校创新基金科研课题(2022B-120) （2022B-120）
甘肃中医药大学引进人才项目(2018YJRC-05) （2018YJRC-05）
