华侨大学学报（自然科学版）2025，Vol.46Issue(4)：435-441,7.DOI:10.11830/ISSN.1000-5013.202412014
C5-12启动子降低rAAV1介导的基因递送后转基因的免疫反应
C5-12 Promoter Reduces Transgene Immune Response Following rAAV1-Mediated Gene Delivery
摘要
Abstract
The efficacy of the C5-12 promoter in reducing or abolishing the immune response against ovalbu-min(OVA)was explored through experiments such as determination of transgene expression,in vitro antigen presentation,and the clearance of transduced positive cells.The experimental results showed that in mouse myoblasts C2C12,the C5-12 promoter had no impact on the expression of OVA,while in mouse bone marrow dendritic cells JAWS Ⅱ,the C5-12 promoter significantly inhibited both the expression of OVA and the pres-entation of OVA antigenic peptides.After muscle administration,the C5-12 promoter could maintain the con-tinuous high expression of OVA in muscle tissues,the humoral immunity remained at a relatively low level,the immune response in muscle tissues was relatively mild,and the clearance of transduced positive cells was significantly reduced.关键词
C5-12启动子/重组腺相关病毒(rAAV)/免疫反应/转基因/卵清蛋白(OVA)Key words
C5-12 promoter/recombinant adeno-associated virus(rAAV)/immune response/transgene/ovalbumin(OVA)分类
生物科学引用本文复制引用
王晓,黄晓平,黎玲,张红,刁勇..C5-12启动子降低rAAV1介导的基因递送后转基因的免疫反应[J].华侨大学学报（自然科学版）,2025,46(4):435-441,7.基金项目
国家自然科学基金资助项目(81502687,81371669) （81502687,81371669）
福建省自然科学基金资助项目(2021J01439) （2021J01439）
福建省泉州市科技计划项目(2020C061) （2020C061）
福建省高等教育协会2022年高等教育科学研究实验室研究专项(22FJSYYB006) （22FJSYYB006）
