华中农业大学学报2025，Vol.44Issue(4)：22-36,15.DOI:10.13300/j.cnki.hnlkxb.2025.04.003
腐殖质氧化还原能力评价方法及其应用
Method for evaluating redox capacity of humic substances and its applications
摘要
Abstract
Humic substances,as a natural organic matter commonly present in soil and sediment,is crucial in the processes of biogeochemical cycling and energy flux within ecosystems.The active functional groups including quinones contained in the molecular structure of humic acid endow humic acid with redox properties,making it play a key role in the transformation of environmental pollutants and the improvement of soil fertility.Accurately evaluating the redox capacity of humic substances is the foundation for the in-depth elucidation of its environmental behavior and functional mechanisms.At present,the main methods for evaluating the redox capacity of humic substances include redox titration,electrochemical techniques,and microbial assays.This article reviewed the principles,experimental protocols,and application scope of oxidation-reduction titration,electrochemical techniques,and microbial assays,and deeply analyzed the ad-vantages and limitations of each method in evaluating the redox capacity of humic substances.The results showed that the redox titration is simple to operate,but its quantitative accuracy is limited by the way of de-termining endpoint.Electrochemical techniques have high sensitivity but are susceptible to external interfer-ence from environments.Microbial assays can simulate natural conditions well but have a longer cycle of analyses.The emerging trends for evaluating the redox capacity of humic substances are discussed in terms of developing new electrochemical sensors,optimizing in-situ measurement techniques,and implementing multi-modal analytical strategies.It will offer a theoretical framework for selecting appropriate methods for evaluating the redox capacity of humic substances from diverse sources and establishing a robust scientific foundation for promoting the application of humic substances in remediating environment and boosting agri-cultural productivity.关键词
腐殖质/有机质/土壤/氧化还原/电子转移Key words
humic substances/organic matter/soil/redox/electron transfer分类
资源环境引用本文复制引用
熊娟,方静,许运,谭文峰..腐殖质氧化还原能力评价方法及其应用[J].华中农业大学学报,2025,44(4):22-36,15.基金项目
国家自然科学基金项目(42277302) （42277302）
