华中农业大学学报2025，Vol.44Issue(4)：150-159,10.DOI:10.13300/j.cnki.hnlkxb.2025.04.015
基于HPLC的轮枝镰刀菌侵染后甘蔗叶片内源激素含量分析
Analysis of endogenous hormone content in sugarcane leaves infected by Fusarium verticillioides based on HPLC
摘要
Abstract
To clarify the relationship between endogenous hormones and resistance to sugarcane pok-kah boeng disease,this study utilized Fusarium verticillioides,the primary pathogenic fungus of sugarcane pokkah boeng disease in China,as the pathogen,and employed the highly resistant cultivar YT94-128(Yu-etan 94-128)and the susceptible cultivar GT37(Guangxi Tang 37)as experimental materials.High-per-formance liquid chromatography(HPLC)was applied to detect the dynamic changes of five endogenous hormones in sugarcane leaves following pathogen inoculation.The results demonstrated that the HPLC method achieved average recovery rates of 99.61%,99.12%,97.36%,96.74%,and 99.90%for gibberellin(GA),jasmonic acid(JA),citric acid(CA),malic acid(MA)and salicylic acid(SA)in sugarcane leaves,respectively.The precision,indicated by relative standard deviation(RSD)values,ranged from 0.033%to 4.220%,highlighting the method's rapid analysis,high resolution,and excellent stability and re-producibility.Post-inoculation analysis revealed distinct hormonal patterns:GA content progressively de-creased in both cultivars during sugarcane growth,with a more pronounced reduction in GT37.JA content initially increased before decling,showing a greater reduction amplitude in GT37.CA content continuously increased in YT94-128 but significantly decreased in GT37.MA content steadily rose and stabilized in YT94-128,whereas GT37 exhibited a peak at 4 days post-inoculation,followed by a stabilization.SA con-tent in both cultivars displayed an initial increase followed by a decline,with more marked fluctuations in YT94-128.This study demonstrates that the differential concentrations and dynamic patterns of endoge-nous hormones following pathogen infection may serve as critical indicators for assessing varietal resistance to sugarcane pokkah boeng disease.关键词
甘蔗/梢腐病/轮枝镰刀菌/内源激素/HPLCKey words
sugarcane/pokkah boeng disease/Fusarium verticillioides/endogenous hormones/high performance liquid chromatography分类
农业科技引用本文复制引用
杨翠芳,高轶静,黄海荣,黄冬梅,杨海,申垚阳,李翔,王泽平..基于HPLC的轮枝镰刀菌侵染后甘蔗叶片内源激素含量分析[J].华中农业大学学报,2025,44(4):150-159,10.基金项目
广西自然科学基金项目(2024GXNSFAA010174) （2024GXNSFAA010174）
国家自然科学基金项目(32260715) （32260715）
国家糖料产业技术体系甘蔗宿根栽培岗位科学家项目(CARS-17) （CARS-17）
蔗糖产业省部共建协同创新中心项目(201812639) （201812639）
南宁市科学与技术研发项目(2023060) （2023060）
