基础医学与临床2025，Vol.45Issue(8)：992-998,7.DOI:10.16352/j.issn.1001-6325.2025.08.0992
基于液质联用技术的尿液蛋白质组学的质量控制
Quality control of urine proteome based on liquid chromatography-mass spectrometry
摘要
Abstract
With the development of automated sample pretreatment technologies and high-throughput mass spec-trometry platforms,large-scale clinical cohort-based proteomic studies have progressively achieved systematic analy-sis at the scale of thousands of samples and tens of thousands of proteins.However,the heterogeneity of instruments across multiple centers may introduce batch effects,systematic bias in long-term experiments,and reliability issues in detecting low-abundance biomarkers,thereby imposing higher demands on the entire workflow quality control system.This review systematically summarizes multidimensional QC parameters including sample preparation,chro-matographic separation,mass spectrometric acquisition and protein identification.In particular,this review focuses on mainstream QC tools for intra-experiment and inter-experiment,emphasizing their primary functions under differ-ent acquisition modes.Finally,it discusses the feasibility of dynamically integrated algorithm-based QC models and multi-omics QC strategies in future research.关键词
蛋白质组学/LC-MS/MS/质量控制/质量控制工具Key words
proteomics/LC-MS/MS/quality control/quality control tools分类
医药卫生引用本文复制引用
刘响,魏静..基于液质联用技术的尿液蛋白质组学的质量控制[J].基础医学与临床,2025,45(8):992-998,7.基金项目
国家自然科学基金青年项目(82301540) （82301540）
