基础医学与临床2025，Vol.45Issue(8)：1010-1015,6.DOI:10.16352/j.issn.1001-6325.2025.08.1010
NF-κB抑制剂或NAC减轻胺碘酮诱导的人肺上皮细胞系BEAS-2B损伤
NF-κB inhibitor or NAC attenuates amiodarone-induced injury of human lung epithelial cell line BEAS-2B
摘要
Abstract
Objective To investigate the mechanism of reactive oxygen species(ROS)and pyroptosis in amioda-rone-induced lung epithelial cell injury.Methods The viability of cells treated with amiodarone,amiodarone+N-acetylcysteine(ROS inhibitor,NAC)and amiodarone+NF-κB inhibitors was detected by MTT assay.ROS level was detected by DCFA probe and the levels of IL-6,IL-18,IL-1β and TNF-α in the supernatant were detected by ELISA;NLRP3.Caspase-1 p20 and GSDMD-N protein were detected by Western blot.Results Amiodarone con-centration-dependently reduced cell viability(P＜0.05),NAC and NF-κB attenuated the cytotoxicity of amiodarone(P＜0.05).Amiodarone increased levels of reactive oxygen species,inflammatory factors and pyroptosis-related proteins in lung epithelial cells(P＜0.05),while NAC and NF-κB inhibitors reduced the levels of these indicators(P＜0.05).Conclusions NF-κB inhibitor or NAC attenuates amiodarone-induced injury of human lung epithelial cell line in vitro.关键词
胺碘酮/活性氧/细胞损伤/NF-κBKey words
amiodarone/reactive oxygen species/cell injury/NF-κB分类
医药卫生引用本文复制引用
计泽朝,张琪,阿不都许库尔·阿不力米提..NF-κB抑制剂或NAC减轻胺碘酮诱导的人肺上皮细胞系BEAS-2B损伤[J].基础医学与临床,2025,45(8):1010-1015,6.基金项目
国家自然科学基金(32060158) （32060158）
