基础医学与临床2025，Vol.45Issue(8)：1028-1033,6.DOI:10.16352/j.issn.1001-6325.2025.08.1028
异丙酚减轻结扎脊神经诱导的大鼠神经病理性疼痛
Propofol alleviates neuropathic pain induced by spinal nerve ligation in rats
摘要
Abstract
Objective To evaluate the improvement effect and mechanism of propofol on neuropathic pain(NP)model rats induced by spinal nerve ligation(SNL).Methods An SNL rat model was established,and 30 success-fully modeled rats were randomly divided into SNL group,propofol+SNL group and propofol+7-N1(7-nitroindazole)+SNL group(n=10 each),with sham group as control(n=10).After propofol intervention,Von Frey method was applied to detect mechanical hypersensitivity in rats.The hot claw foot analgesic device was applied to detect the la-tent period of the heat contraction foot reflex.Enzyme-linked immunosorbent assay(ELISA)method was applied to detect the expression level of interleukin-6(IL-6)and interferon-γ(IFN-γ)in the spinal cord of rats.RT-qPCR method was used to detect the mRNA expressions of suppressor of cytokine signaling 3(SOCS3),arginase-1(Arg-1)and cluster of differentiation 68(CD68)in the spinal dorsal horn.Western blot was applied to detect the expression level of proteins related to the nitric oxide/cyclic guanosine monophosphate(NO/cGMP)signaling pathway.Results Compared with the sham group,the mechanical foot contraction reflex threshold,thermal foot contraction reflex latency,and the expression level of Arg-1 mRNA,endothelial nitric oxide synthase(eNOS)and soluble gua-nylate cyclase(sGCa)proteins were significantly reduced in the SNL group(P＜0.05),the level of IL-6 and IFN-γ and the expression level of SOCS3,CD68 mRNA and inducible nitric oxide synthase(iNOS)protein were signifi-cantly increased(P＜0.05).Compared with the SNL group,the mechanical foot reflex threshold,thermal foot reflex latency of rats in the propofol+SNL group,the expression level of Arg-1 mRNA,eNOS and sGCa protein were sig-nificantly increased(P＜0.05),the level of IL-6 and IFN-γ and the expression of SOCS3,CD68 mRNA and iNOS protein were all decreased(P＜0.05).The NO/cGMP signaling pathway inhibitor 7-N1 reversed the improvement effect of propofol on SNL rats(P＜0.05).Conclusions Propofol alleviates NP and injury in SNL rats by activating the NO/cGMP signaling pathway.关键词
异丙酚/脊神经结扎/神经病理性疼痛/NO/cGMP信号通路Key words
propofol/spinal nerve ligation/neuropathic pain/NO/cGMP signaling pathway分类
医药卫生引用本文复制引用
高彦东,边步荣,王波..异丙酚减轻结扎脊神经诱导的大鼠神经病理性疼痛[J].基础医学与临床,2025,45(8):1028-1033,6.基金项目
陕西省自然科学基础研究计划项目(2019JQ-983) （2019JQ-983）
