解放军医学杂志2025，Vol.50Issue(6)：716-720,5.DOI:10.11855/j.issn.0577-7402.0914.2025.0209
抗生素疗程对早产儿日龄28d时肠道菌群及免疫功能的影响
Effects of different courses of antibiotics on intestinal floras and immune function in 28-day-old premature infants
摘要
Abstract
Objective To investigate the impact of different antibiotic courses on intestinal floras and immune function in 28-day-old premature infants.Methods A retrospective analysis was conducted on 120 premature infants admitted to the First People's Hospital of Nanyang City between March 2021 and March 2024.According to different courses of antibiotics,they were divided into short-course group(n=82,antibiotics use≤3 d)and long-course group(n=38,antibiotics use≥7 d).General data were collected,and intestinal floras and immune function were assessed at 28 days of age.Shannon and Simpson indexes of intestinal flora α-diversity,bacterial phylum and genus distribution of intestinal floras,and peripheral blood CD8+,CD4+T cell percentages,and CD4+/CD8+ratio were compared between the two groups.Pearson correlation analysis was used to explore the relationship between intestinal floras and immune function,and adverse outcomes were recorded.Results The Shannon and Simpson indexes of intestinal flora α-diversity in long-course group were lower than those in short-course group(P<0.05).No significant differences were found between the two groups in the distribution of Actinobacteria,Bacteroidetes,Firmicutes or Proteobacteria at the bacterial phylum level and Bacteroides,Enterococcus,or Veillonella at genus distribution level(P>0.05).However,the long-course group had lower proportions of Clostridioides,Clostridium sensu stricto 1,Escherichia and Klebsiella,and higher proportions of Enterococcus,Robinsoniella and Streptococcus than those in short-course group(P<0.05).After antibiotics use,levels of CD4+and CD4+/CD8+were decreased(P<0.05),while CD8+was increased in both groups(P<0.05).Compared with short-course group,levels of CD4+and CD4+/CD8+were lower,while CD8+was higher in short-course group(P<0.05).Pearson correlation analysis revealed that Clostridioides,Clostridium sensu stricto 1,Escherichia,Klebsiella were positively correlated with CD4+and CD4+/CD8+,while negatively correlated with CD8+(P<0.05).Conversely,Enterococcus,Robinsoniella and Streptococcus were negatively correlated with CD4+and CD4+/CD8+,while positively correlated with CD8+(P<0.05).No adverse outcomes occurred in two groups.Conclusion Compared with short-term antibiotic use,long-term use affects the colonization and diversity of intestinal floras in premature infants,and cause intestinal flora disturbance,potentially impairing immune function.关键词
抗生素/疗程/早产儿/肠道菌群/免疫功能Key words
antibiotic/course of treatment/premature infant/intestinal flora/immune function分类
医药卫生引用本文复制引用
李莹,黄玉焕,张红阳,张靖..抗生素疗程对早产儿日龄28d时肠道菌群及免疫功能的影响[J].解放军医学杂志,2025,50(6):716-720,5.基金项目
This work was supported by the Henan Provincial Medical Science and Technology Research Project(LHGJ20191453) 河南省医学科技攻关计划联合共建项目(LHGJ20191453) （LHGJ20191453）
