解剖学杂志2025，Vol.48Issue(3)：209-214,6.DOI:10.3969/j.issn.1001-1633.2025.03.005
LncRNA CASC9/miR-874-3p/ADAM19轴调控视网膜母细胞瘤细胞增殖、迁移和侵袭
LncRNA CASC9/miR-874-3p/ADAM19 axis regulates proliferation,migration,and invasion of retinoblastoma cells
摘要
Abstract
Objective:To investigate the effect of long non-coding RNA(lncRNA)cancer susceptibility candidate 9(CASC9)/miR-874-3p/a disintegrin and metalloproteinase 19(ADAM19)axis on the proliferation,migration and invasion of retinoblastoma(RB)cells.Methods:Real-time fluorescence quantitative PCR method was applied to detect the expression levels of CASC9,miR-874-3p,and ADAM19 mRNA in RB tumor tissues and cells.Immunohistochemistry was used to detect the expression of ADAM19 protein.CCK-8 method and Transwell assay were applied to evaluate the cell proliferation,migration,and invasion.Western blotting was used to detect the expression of E-cadherin,N-cadherin,Vimentin and ADAM19 proteins.Dual-luciferase reporter assays were employed to verify the relationship between miR-874-3p and CASC9 or ADAM19.Results:CASC9 and ADAM19 were highly expressed in RB tumor tissues,while miR-874-3p showed low expression.Knockdown of CASC9 inhibits cell proliferation,migration,and invasion,and reduced the expressions of ADAM19,N-cadherin and Vimentin,while promoting the expression levels of miR-874-3p and E-cadherin.Inhibition of miR-874-3p weakened the effects of knockdown of CASC9 on cell proliferation,migration,invasion and protein expression.CASC9 targeted and negatively regulated miR-874-3p expression,while miR-874-3p targeted and negatively regulated ADAM19 expression.Conclusion:Knocking down CASC9 may inhibit the expression of ADAM19 protein by up-regulating miR-874-3p,thereby inhibiting the proliferation,migration,and invasion of RB cells.关键词
长链非编码RNA癌症易感性候选基因9/miR-874-3p/解整合素金属蛋白酶19/视网膜母细胞瘤/迁移Key words
long non-coding RNA cancer susceptibility candidate 9/miR-874-3p/ADAM19/retinoblastoma/migration分类
医药卫生引用本文复制引用
尹双慧,严肖啸,孙敏..LncRNA CASC9/miR-874-3p/ADAM19轴调控视网膜母细胞瘤细胞增殖、迁移和侵袭[J].解剖学杂志,2025,48(3):209-214,6.基金项目
河北省2023年度医学科学研究课题计划(20231955) （20231955）
