解剖学杂志2025，Vol.48Issue(3)：221-227,7.DOI:10.3969/j.issn.1001-1633.2025.03.007
LINC01510通过靶向细胞周期蛋白依赖性激酶14促进骨肉瘤细胞的恶性进展
LINC01510 promotes malignant progression of osteosarcoma cells by targeting cyclin-dependent kinase 14
摘要
Abstract
Objective:To investigate the molecular mechanism by which the long non-coding RNA(lncRNA)LINC01510 promotes the proliferation,metastasis and invasion of osteosarcoma cells by targeting cyclin-dependent kinase 14(CDK14).Methods:The differences in expression of LINC01510 and CDK14 between normal and tumor tissues of osteosarcoma patients,and between human normal osteoblasts and osteosarcoma cell lines were investigated by RT-qPCR and Western blotting.Cell transfection,SRB staining,and colony formation assays were performed to investigate the effects of LINC01510 knockdown on osteosarcoma cell proliferation.The impact of LINC01510 knockdown on the cell metastasis and invasion of osteosarcoma cells was assessed by using cell transfection techniques and Transwell assays.Bioinformatics tools and methods were employed to identify the miRNAs that interact with LINC01510 and CDK14.RNA immunoprecipitation assays and RT-qPCR techniques were utilized to examine the binding interactions between LINC01510,CDK14 and miR-339-5p.The dual luciferase reporter gene system,cell transfection technique and RT-qPCR technique were adopted to examine the regulatory mechanisms of LINC01510,CDK14 and miR-339-5p.Results:The expression levels of LINC01510 and CDK14 were significantly upregulated in patients'osteosarcoma tissues and immortalized osteosarcoma cell lines.Knockdown of LINC01510 inhibited the proliferation,metastasis and invasion of osteosarcoma cells.LINC01510 knockdown suppressed CDK14 expression by up-regulating miR-339-5p targeting the 3'-UTR of CDK14.LINC01510/miR-339-5p/CDK14 signaling axis regulated proliferation,invasion and metastasis of osteosarcoma cells.Conclusion:LINC01510 regulates CDK14 expression by targeting the 3'-UTR region via miR-339-5p,eventually regulating the proliferation,invasion and metastasis of osteosarcoma cells.关键词
非编码RNA/骨肉瘤/LINC01510/细胞周期蛋白依赖性激酶14/miR-339-5pKey words
non-coding RNA/osteosarcoma/LINC01510/cyclin-dependent kinase 14/miR-339-5p分类
医药卫生引用本文复制引用
刘德国,徐天波,吴良邦,吕骜,郑宇翔,侯振海..LINC01510通过靶向细胞周期蛋白依赖性激酶14促进骨肉瘤细胞的恶性进展[J].解剖学杂志,2025,48(3):221-227,7.基金项目
浙江省教育厅一般科研项目(Y202249274) （Y202249274）
