解剖学杂志2025，Vol.48Issue(3)：228-234,7.DOI:10.3969/j.issn.1001-1633.2025.03.008
丙泊酚通过微小RNA-613调控胃癌细胞增殖、迁移和侵袭的机制
Mechanism of propofol in regulating proliferation,migration,and invasion of gastric cancer cells via microRNA-613
摘要
Abstract
Objective:To investigate the effects of propofol on the proliferation,migration and invasion of gastric cancer cells by targeting glucose-6-phosphate dehydrogenase(G6PD)through microRNA-613(miR-613).Methods:Gastric cancer cells SGC7901 were cultured in vitro and divided into control group,propofol group,propofol+inhibitor NC group,propofol+miR-613 inhibitor group,propofol+miR-613 inhibitor+si-NC group,and propofol+miR-613 inhibitor+si-G6PD group.Real-time fluorescence quantitative PCR(RT-qPCR)was applied to detect the miR-613 expression.CCK-8 experiment was applied to detect cell proliferation.Flow cytometry was employed to detect cell apoptosis.Transwell assay was adopted to detect cell migration and invasion.Western blotting was performed to detect the expression of G6PD and epithelial mesenchymal transition(EMT)marker proteins,and dual-luciferase reporter assay was utilized to confirm the targeting relationship between miR-613 and G6PD.Results:Compared with the control group,the numbers of SGC7901 cell migration and invasion was decreased significantly,along with a significant decrease in OD450 value,G6PD,and N-cadherin expression in the propofol group,while the apoptosis rate,miR-613,and E-cadherin expression were obviously increased.Inhibiting the expression of miR-613 weakened the inhibitory effect of propofol on the proliferation,migration,and invasion of gastric cancer cells.The low expression of G6PD reversed the promoting effect of miR-613 silencing on the proliferation,invasion,and migration of gastric cancer cells.Conclusion:Propofol may inhibit the proliferation,migration,and invasion of gastric cancer cells by targeting G6PD through miR-613.关键词
胃癌/丙泊酚/miR-613/葡萄糖-6-磷酸脱氢酶/增殖/迁移/侵袭Key words
gastric cancer/propofol/miR-613/glucose 6-phosphate dehydrogenase/proliferation/migration/invasion分类
医药卫生引用本文复制引用
史国强,谷甫艳,牛卫康,李喜龙..丙泊酚通过微小RNA-613调控胃癌细胞增殖、迁移和侵袭的机制[J].解剖学杂志,2025,48(3):228-234,7.基金项目
河南省医学科技攻关项目(LHGJ20200184) （LHGJ20200184）
