摘要
Abstract
Objective:To investigate the effect of fasudil on the proliferation,apoptosis and angiogenesis of bladder cancer cells by regulating RhoA/ROCK signaling pathway.Methods:T24 cells of bladder cancer were divided into control group,low-dose fasudil group,medium-dose fasudil group,high-dose fasudil group,and high-dose fasudil+Naciclassine(RhoA activator)group.MTT and EdU assays were adopted to detect cell proliferation.Transwell assay was applied to detect cell migration and invasion.Flow cytometry was employed to detect cell apoptosis rate.Three-dimensional Matrigel culture was performed to observe angiogenesis.Western blotting was utilized to detect the expression levels of vascular endothelial growth factor A(VEGF-A),vascular endothelial cadherin(VE cadherin),Ki-67,MMP-9,caspase-3,RhoA,and ROCK proteins in cells.Results:The control group cells had good lumen structure.Compared with the control group,the lumen structure of T24 cells in the low,medium,and high-dose fasudil groups showed varying degrees of damage,and the OD490 values(at 24 h and 48 h),cell viability,numbers of cell migration and invasion,the expression of VEGF-A,VE cadherin,Ki-67,MMP-9,RhoA,and ROCK proteins were obviously reduced,while the apoptosis rate and the expression of caspase-3 protein were obviously increased.Naciclassine partially reversed the inhibitory effect of fasudil on T24 cells.Conclusion:Fasudil can inhibit the malignant biological behavior of bladder cancer cells,possibly by blocking the RhoA/ROCK signaling pathway activation.关键词
法舒地尔/RhoA/ROCK/膀胱癌/血管生成Key words
fasudil/RhoA/ROCK/bladder cancer/angiogenesis分类
医药卫生
