江苏大学学报（医学版）2025，Vol.35Issue(4)：318-325,8.DOI:10.13312/j.issn.1671-7783.y240040
甲基转移酶SETD7促进卵巢癌细胞增殖和迁移
Methyltransferase SETD7 promotes the proliferation and migration of ovarian cancer cells
摘要
Abstract
Objective:To investigate the expression of SET domain containing lysine methyltransferase 7(SETD7)in ovarian cancer and its effect on epithelial-mesenchymal transition(EMT),as well as the proliferation and migration of ovarian cancer cells.Methods:The expression level of SETD7 in ovarian cancer microarrays was detected by immunohistochemical staining,and the correlation between SETD7 expression and clinicopathological features and prognosis of ovarian cancer patients was analyzed.Both stable knockdown SETD7 and overexpression SETD7 cell lines were constructed by using lentiviral expression system;cell counting,CCK-8,Transwell and scratch assay were used to evaluate the proliferation and migration ability of ovarian cancer cells,while Western blotting was used to determine the expression levels of EMT-related proteins.Results:Compared with normal ovarian tissues,the expression level of SETD7 protein in human ovarian cancer tissues was significantly up-regulated(P<0.01).High expression of SETD7 was greatly correlated with M stage,FIGO stage and progression-free survival in ovarian cancer patients(P<0.05).Knockdown of SETD7 significantly inhibited the proliferation and migration of ovarian cancer A2780 cells(P<0.05),while overexpression was the opposite in SKOV3 cells.Overexpression of SETD7 up-regulated the expression of N-cadherin and Snail,and down-regulated the expression of E-cadherin,while its knockdown exerted the opposite in A2780 cells.Conclusion:SETD7 is up-regulated in human ovarian cancer tissues and cells,which promotes the migration,proliferation and EMT process of ovarian cancer cells.关键词
卵巢癌/含有SET结构域的赖氨酸甲基转移酶7(SETD7)/增殖/迁移/上皮-间质转化Key words
ovarian cancer/SET domain containing lysine methyltransferase 7(SETD7)/proliferation/migration/epithelial-mesenchymal transition分类
医药卫生引用本文复制引用
张紫薇,侯雅楠,黄婷,张博文,李明阳,林琼,邵根宝..甲基转移酶SETD7促进卵巢癌细胞增殖和迁移[J].江苏大学学报（医学版）,2025,35(4):318-325,8.基金项目
江苏省重点研发计划(BE2020678) （BE2020678）
