实用临床医药杂志2025，Vol.29Issue(12)：19-25,7.DOI:10.7619/jcmp.20250239
中波紫外线辐射对人角膜上皮细胞和兔角膜损伤效应的研究
Effects of ultraviolet B radiation on impairment of human corneal epithelial cells and rabbit cornea
摘要
Abstract
Objective To compare the changes in biological indicators of human corneal epithe-lial(HCET)cells and rabbit corneas after exposure to different doses of ultraviolet B(UVB)radia-tion,so as to evaluate the impact of UVB radiation on corneal injury effects.Methods In cell exper-iment,HCET cells were divided into groups with radiation doses of 0,6,12,18,and 24 mJ/cm2.The effect of UVB radiation on HCET cell viability was detected using the CCK-8 assay,and the level of intracellular DNA damage was assessed by immunofluorescence.In the animal experiment,15 healthy New Zealand white rabbits(30 eyes)were randomly divided into groups with radiation doses of 0,1.35,2.16,4.32,and 6.48 J/cm2.The UVB exposure time for the radiation groups was 30 minutes per day for 3 consecutive days.Corneal injury was evaluated using methods such as slit-lamp microscopy,sodium fluorescein staining,central corneal thickness measurement,optical coherence tomography(OCT)imaging,and hematoxylin and eosin(HE)staining.Results Compared with the control group,cell viability in the radiation groups gradually decreased,and the level of DNA damage gradually increased with increasing radiation dose.As the radiation dose increased in the radiation groups,the degree of corneal opacity in rabbits gradually worsened,the central corneal area gradu-ally thickened,and OCT revealed high-intensity scattered light signals with the formation of shadow areas.Results from HE staining,immunohistochemistry,Western blot(WB),and sodium fluores-cein staining showed that the 1.35 J/cm2 group caused mild corneal injury,with damage reaching the corneal epithelial layer.In the 2.16 J/cm2 group,the corneal injury presented as dense punctate distribution,with damage extending from the epithelial layer to the superficial stroma.The number of ephrin type-A receptor 2(EphA2)protein-stained cells was relatively small,and the staining was light,showing a weak positive result.In the 4.32 J/cm2 and 6.48 J/cm2 groups,the corneal injury was irreversible,with damage gradually progressing from the corneal epithelial layer and superficial stroma to the endothelial layer.The number of EphA2 protein-stained cells was relatively large,and the staining was dark,showing a strong positive result.Conclusion This study comprehensively e-valuates the dose-dependent injury effects of UVB on HCET cells and New Zealand white rabbit cor-neas through cell and animal experiments.It elucidates that UVB radiation could induce corneal cell DNA damage,promote inflammatory responses,and trigger apoptosis by upregulating γ-phosphoryla-ted histone H2AX(γH2AX)and EphA2.The self-repair ability and process of corneal injury are preliminarily explored,providing a basis for further research on mechanisms of corneal injury caused by ultraviolet radiation and the development of protective drugs.关键词
中波紫外线/眼角膜损伤/角膜上皮细胞/氧化应激/线粒体损伤/DNA损伤/促红细胞生成素肝细胞受体A2/γ-磷酸化组蛋白H2AXKey words
ultraviolet B/corneal injury/corneal epithelial cells/oxidative stress/mito-chondrial damage/DNA damage/erythropoietin-producing hepatocyte receptor A2/γ-phosphoryla-ted histone H2AX分类
医药卫生引用本文复制引用
崔名慧,于豪冰,朱燊,王心园,王杨凯,刘小宇,胡波..中波紫外线辐射对人角膜上皮细胞和兔角膜损伤效应的研究[J].实用临床医药杂志,2025,29(12):19-25,7.基金项目
国家重点研发计划项目(2022YFC2804500) （2022YFC2804500）
