江苏农业学报2025，Vol.41Issue(6)：1041-1049,9.DOI:10.3969/j.issn.1000-4440.2025.06.001
小麦TaLFNR1-7A基因的生物信息学及表达分析
Bioinformatics and expression analysis of TaLFNR1-7A gene in wheat
摘要
Abstract
Ferritin-NADP+ reductase(FNR)is an important enzyme that plays a crucial role in photosynthesis and cellular respiration processes.This study used common wheat Chinese Spring as the material and cloned the TaLFNR1-7A gene using RT-PCR technology.Bioinformatics and gene expression analysis were performed on it.The results showed that the CDS sequence length of TaLFNR1-7A gene was 1 086 bp,encoding 361 amino acids.The encoded protein was a non-transmembrane stable hydrophilic protein,without a signal peptide,containing a total of 39 phosphorylation sites.It was predicted to be localized in the chloroplast.Phylogenetic analysis and multiple amino acid sequence alignment revealed that common wheat(Chinese Spring)and emmer wheat exhibited closer phylogenetic proximity based on the TaLFNR1-7A gene,with a sequence similarity of 97.01％.Analysis of promoter cis-acting elements revealed that the TaLFNR1-7A gene contained 17 cis-acting elements,including seven light-responsive elements.The qRT-PCR analysis revealed that the ex-pression of TaLFNR1-7A gene was highest in wheat leaves,followed by stems and germs,with the lowest level observed in roots.The TaLFNR1-7A gene responded to multiple abiotic stresses,including drought(PEG-6000 treatment),methyl jasmonate(MeJA),sodium chloride(NaCl),and abscisic acid(ABA).The findings of this study provide a significant foundation for further exploring the functional mechanisms of the TaLFNR1-7A gene in wheat growth and development.关键词
小麦/TaLFNR1-7A基因/生物信息学/基因克隆/表达分析Key words
wheat/TaLFNR1-7A gene/bioinfor-matics/gene cloning/expression analysis分类
农业科技引用本文复制引用
邓云颢,徐灵,李鲁华,宋婵,徐如宏..小麦TaLFNR1-7A基因的生物信息学及表达分析[J].江苏农业学报,2025,41(6):1041-1049,9.基金项目
国家自然科学基金项目(32360474、32160456、32360486) （32360474、32160456、32360486）
贵州省科技支撑计划项目[黔科合支撑(2021)一般272] （2021）
贵州省粮油作物分子育种重点实验室项目[黔科合中引地(2023)008] （2023）
贵州省高等学校功能农业重点实验室项目[黔教技(2023)007号] （2023）
