江苏农业学报2025，Vol.41Issue(6)：1095-1106,12.DOI:10.3969/j.issn.1000-4440.2025.06.006
半夏ARF转录因子家族的全基因组鉴定及生物信息学分析
Genome-wide identification and bioinformatics analysis of the ARF tran-scription factor family in Pinellia ternata
摘要
Abstract
Pinellia ternata is an important medicinal plant.During its growth,it is extremely sensitive to light and temperature,and prone to the phenomenon of seedling collapse,which leads to a reduction in yield.In this study,based on the genomic data of Pinellia ternata released by the China National Center for Bioinformation,25 members of the PtARF family were identified and divided into four sub-families through phylogenetic analysis.The results of protein physicochemi-cal property analysis showed that all PtARFs in Pinellia ternata were hydrophilic and unstable proteins,and most PtARFs were located in the cell nucleus.The prediction of the protein tertiary structure indicated that the tertiary structures of PtARF family members with close genetic relationships were highly similar,suggesting potential functional redundancy among some PtARF proteins.The results of phylogenetic analysis and collinearity analysis showed that the ARF genes of Pinellia ternata and rice were closely re-lated.In the promoter regions of the ARF family genes in Pinellia ternata,the cis-acting elements with the largest number were those related to biotic and abiotic stress re-sponses,followed by plant hormone-responsive elements,and the elements related to plant growth and development responses were the least in number.This result indicated that PtARFs may be widely involved in the response of Pinellia ternata to stress.Further research results showed that PtARF could respond to low-light stress and high-temperature stress,and may be involved in the regulation of seedling collapse and tuber development in Pinellia ternata.This study provides an important theoretical basis for stress-resistant breeding and op-timization of cultivation techniques of Pinellia ternata.关键词
半夏/ARF转录因子家族/倒苗/生物信息学Key words
Pinellia ternate/ARF transcription factor family/sprout tumble/bioinformatics分类
农业科技引用本文复制引用
朱志炎,张德清,周厚英,何勇,田志宏..半夏ARF转录因子家族的全基因组鉴定及生物信息学分析[J].江苏农业学报,2025,41(6):1095-1106,12.基金项目
江苏省高等学校基础科学(自然科学)研究面上项目(24KJD210003) （自然科学）
南通师范高等专科学校青年项目(TSGZ2022Q02) （TSGZ2022Q02）
湿地生态与农业利用教育部工程研究中心开放基金项目(KF202321) （KF202321）
