江苏农业学报2025，Vol.41Issue(6)：1107-1115,9.DOI:10.3969/j.issn.1000-4440.2025.06.007
百香果果生炭疽菌小GTP酶基因Rab7的鉴定与功能分析
Identification and functional analysis of the small GTPase gene Rab7 in Colletotrichum fructicola of Passiflora edulis
摘要
Abstract
Anthracnose of Passiflora edulis caused by Colletotrichum fructicola is a significant disease affecting the yield and quality of passion fruit.Elucidating the pathogenic molecular mechanisms of Colletotrichum fructicola is of great significance for disease control.In this study,we identified the small GTPase Rab7 from C.fructicola and compared its amino acid sequences with those of Rab7 proteins from various fungi.Phylogenetic analysis revealed that the Rab7 protein of C.fructicola had the most distant relationship with the Rab7 protein of Saccharomyces cerevisiae,while it was closely related to the Rab7 proteins of Colleto-trichum gloeosporioides and Colletotrichum graminicola.Using homologous recombination technology,we constructed the Rab7 gene knockout strain △Rab7 and the complemented strain △Rab7/Rab7.Phenotypic analysis showed that com-pared with the wild type and △Rab7/Rab7,the △Rab7 mu-tant exhibited significantly reduced colony diameter on PDAY and MM media,significantly increased melanin accumula-tion,and significantly decreased conidial production.However,△ Rab7 and the wild type N425 did not show significant differ-ences in sensitivity to H2O2 stress.Confocal laser scanning microscopy observations indicated that the Rab7-GFP fusion protein was localized to the vacuolar membrane,and the vacuoles in △Rab7 appeared fragmented,suggesting that Rab7 gene maintained normal vacuolar morphology in C.frructicola by regulating vacuolar fusion.This study provides a theoretical basis for elucidating the molecular mechanisms of pathogenicity in C.frructicola.关键词
百香果/果生炭疽菌/小GTP酶基因Rab7/致病性Key words
Passiflora edulis/Colletotrichum fructicola/small GTPase gene Rab7/pathogenicity分类
农业科技引用本文复制引用
张承康,黄欣,李希荣,郭彦超,郭田龙,陈美霞..百香果果生炭疽菌小GTP酶基因Rab7的鉴定与功能分析[J].江苏农业学报,2025,41(6):1107-1115,9.基金项目
福建省自然科学基金项目(2021J05254) （2021J05254）
宁德师范学院创新团队项目(2022T01) （2022T01）
