江苏农业学报2025，Vol.41Issue(6)：1188-1196,9.DOI:10.3969/j.issn.1000-4440.2025.06.015
江苏省鸡源沙门菌的分子流行病学特征分析
Molecular epidemiological characteristics of Salmonella isolated from chickens in Jiangsu province
摘要
Abstract
In order to clarify the molecular epidemiological characteristics of Salmonella from chickens in Jiangsu prov-ince,1 641 fecal samples of sick chickens from large-scale chicken farms in eight cities of Jiangsu province were collected to iso-late Salmonella,and the sensitivity of Salmonella isolates to antibiotics was determined by microbroth dilution method.Based on the whole genome sequencing technology,the carrying status of drug resistance genes and virulence genes of the isolates was ana-lyzed.The serotype of the isolates was determined by identifying the antigenic structure of Salmonella,and the epidemiological characteristics of Salmonella in Jiangsu province were analyzed by multilocus sequence typing(MLST)and phylogenetic tree.The results showed that 266 strains of Salmonella were isolated from 1 641 chicken manure samples,and the isolation rate was 16.21％.The resistance rates of the isolated strains to ampicillin and sulfamethoxazole were 56.39％and 55.64％,respectively.Multi-drug resistant bacteria accounted for 53.38％.The proportion of strains carrying sul2,blaTEM-1 and tetA genes was 59.40％,56.77％and 39.10％,respectively.A total of 21 ST types were identified by MLST,of which ST11 accounted for 49.62％.The serotypes were mainly enteritis,pullorum dis-ease,typhoid fever and Indiana.The Salmonella isolates were divided into five genetic evolution branches.The genetic evo-lution relationship between the same farm isolates was rela-tively close,and the genetic relationship between different farm isolates was far.The results of this study provide a basis for the precise prevention and control of Salmonella in chickens in Jiangsu province.关键词
鸡源沙门菌/流行病学/血清型/多位点序列分型/耐药基因/毒力基因Key words
Salmonella isolated from chickens/epidemiology/serotypes/multilocus sequence typing/resistance genes/virulence genes分类
农业科技引用本文复制引用
苏韦赫,吉星,张莉莉,王佳芸,何涛,王冉..江苏省鸡源沙门菌的分子流行病学特征分析[J].江苏农业学报,2025,41(6):1188-1196,9.基金项目
"十四五"国家重点研发计划项目(2023YFD1800303) （2023YFD1800303）
