江苏农业学报2025，Vol.41Issue(6)：1197-1206,10.DOI:10.3969/j.issn.1000-4440.2025.06.016
四种光唇鱼属鱼类线粒体DNA全序列测定及分析
Complete sequence determination and analysis of mitogenome of four spe-cies in Acrossocheilus
摘要
Abstract
In this study,samples were collected and mitochondrial DNA sequences were measured from Acrossocheilus jishouensis,A.parallens,A.spinifer and A.iridescens.The complete mtDNA sequences of four species were obtained through DNAstar Lasergene 7.0 and MEGA7.0 softwares.The protein-coding genes were located by MITOSWebServer online software,and the transfer RNA(tRNA)genes were identified and analyzed by tRNAscan-SE 2.0.The ribosomal RNA(rRNA)genes and control regions were identified by BLAST homology analysis.Incorporating oth-er Acrossochelius species and selected representatives of the subfamily Barbinae available in GenBank,phylogenetic trees were constructed to analyze the phylogenetic relationships of the four Acrossochelius species.The nucleotide components of mtDNA of the four species were similar.The sequences were 16 585-16 595 bp in length,including 37 coding genes and one non-coding region(control region).Eight tRNA genes(tRNAGln,tRNAAla,tRNAAsn,tRNACys,tRNATyr,tRNASer,tRNAGlu,tRNAPro)and ND6 were located on the light strand(L strand),and the remaining genes were located on the heavy strand(H strand).Among the 13 protein-coding genes,the ND2 gene of A.spinifer used ATC as the start codon,which was different from the other three species.The start codons of ND3 genes of A.parallens and A.spinifer were GTG,while the other two species used ATG as the start codon,and the start codons of the remaining genes were ATG or GTG.There were four types of stop codons(TAA,TAG,TA and T).All 22 tRNA genes encoded tRNAs that possessed the typi-cal cloverleaf secondary structure,except for the tRNA encoded by the tRNASer gene,which lacked the DHU arm.The vari-ation of control region was obvious,but the corresponding termination sequence region,central conserved region and con-served sequence region were identified in control region.Phylogenetic analysis showed that the Acrossocheilus species could be divided into two clades.A.parallens was most closely related to A.jishouensis and most distantly related to A.iridescens.By systematically analyzing the complete mitochondrial DNA(mtDNA)sequences of four Acrossochelius species,this study elucidates their genome structure,characteristics,and taxonomic status.These findings provide a theoretical foundation for the phylogenetic analysis of the genus Acrossochelius and the conservation of its germplasm resources.关键词
吉首光唇鱼/侧条光唇鱼/厚刺光唇鱼/虹彩光唇鱼/线粒体基因组/系统发育分析Key words
Acrossocheilus jishouensis/Acrossocheilus parallens/Acrossocheilus spinifer/Acrossocheilus iridescens/mitogenome/phylogenetic analysis分类
农业科技引用本文复制引用
梁巍骞,李强,刘兰苑,陈锭娴,王凯丰,陈炜坚,林胜跃,叶树政,龚剑,桂林..四种光唇鱼属鱼类线粒体DNA全序列测定及分析[J].江苏农业学报,2025,41(6):1197-1206,10.基金项目
国家自然科学基金项目(41673110) （41673110）
广州市科技计划项目(201804010486) （201804010486）
广东省教育科学规划课题(2021GXJK097) （2021GXJK097）
