菌物学报2025，Vol.44Issue(7)：120-129,10.DOI:10.13346/j.mycosystema.250094
野生虎乳木孔菌生物学特性及其驯化栽培
Biological characteristics and domestication cultivation of Lignosus rhinocerus
摘要
Abstract
A wild Lignosus strain EPPI-2021-159 isolated from Baoting Li and Miao Autonomous County of Hainan Province was identified as Lignosus rhinocerus through morphological characterization and ITS sequence analysis.The optimal carbon source,nitrogen source,temperature,and pH for mycelial growth were determined using orthogonal experimental design.The results showed that sucrose was the optimal carbon source and yeast extract powder was the optimal nitrogen source,and the strain grew well at 28℃ and pH 5.0.Domestication trials revealed bagful mycelial colonization time in substrate containing 45％sawdust was(45.65±2.32)days,producing sclerotia averaging(149.73±8.90)g fresh weight.This study provides foundational data supporting domestication cultivation of the fungus on a large scale.关键词
虎乳木孔菌/菌核/生物学特性/驯化栽培Key words
Lignosus rhinocerus/sclerotium/biological characteristics/domestication cultivation引用本文复制引用
程佳欣,徐林,丁野,张凯鹏,杨阳,程汉亭,步连燕,李勤奋..野生虎乳木孔菌生物学特性及其驯化栽培[J].菌物学报,2025,44(7):120-129,10.基金项目
海南省重点研发计划(ZDYF2023XDNY065,ZDYF2025XDNY124)This work was supported by the Science and Technology Special Fund of Hainan Province(ZDYF2023XDNY065,ZDYF2025XDNY124). （ZDYF2023XDNY065,ZDYF2025XDNY124）
