江西农业大学学报2025，Vol.47Issue(4)：855-864,10.DOI:10.3724/aauj.2025073
基于高密度SNP Bin标记和SSR标记的遗传图谱鉴定东乡野生稻抽穗期QTL
Identification of heading date QTLs in Dongxiang wild rice using high density SNP bin and SSR markers-based genetic map
摘要
Abstract
[Objective]Heading date(HD)is an important trait which determines the planting season,regional adaptability and yield in rice.It is great significance to explore quantitative trait loci(QTLs)and candidate genes controlling heading date from Dongxiang wild rice(DXWR)for the study of genetic mechanism,variety improvement and domestication.[Method]One BC1F12 backcross recombinant inbred line(BIL)L5339 derived from the interspecific cross between Dongxiang wild rice and XieqingzaoB(XB)was backcrossed with XB to generate 19H19 at BC4F2.Then,two populations,including 23A population at BC5F2 and DX population at BC5F3 were constructed by crossing 19H19 with XB.The QTLs for HD under early season environment(E1)was detected using 23A population and high density genetic map containing 2059 Bin markers,whereas QTLs under middle season(E2)and late season(E3)were mapped using DX population and 182 SSR markers.[Result]A total of 9 QTLs of heading date were detected on chromosomes1,2,6,7,8 and 10 under E1,under E2 and E3 environments.Among them,qHD1.1 was stably detected in both E1 and E2 environments,with DXWR alleles delaying the heading date by 0.98 d and 2.87 d,respectively.In comparison with the physical position of previous HD QTL and genes,eight QTLs(qHD1.1,qHD1.2,qHD6.1,qHD6.2,qHD6.3,qHD7,qHD8 and qHD10)were found to be located in the same regions,while qHD2 has not been cloned.[Conclusion]qHD1.1 was stably detected in both early-and middle-season rice environments and deserves further study.Meanwhile,the locus delays heading date and enhances panicle length,number of spikelet per panicle,and number of grains per panicle in both early-and middle-season environments,which would be beneficial for the genetic improvement of high-yield early-and middle-season rice varieties and the breeding application of HD genes from Dongxiang wild rice.关键词
东乡野生稻/抽穗期/QTL定位/序列分析/多效性Key words
Dongxiang wild rice/heading date/QTL mapping/sequencing analysis/pleiotropic effects分类
农业科技引用本文复制引用
李霞,王世林,吴婷,汪火林,周诗琪,胡标林..基于高密度SNP Bin标记和SSR标记的遗传图谱鉴定东乡野生稻抽穗期QTL[J].江西农业大学学报,2025,47(4):855-864,10.基金项目
江西省重大科技研发专项(20232ACF01001)和江西省自然科学基金重点项目(20224ACB205001) Project supported by the Major Scientific Research and Development Project of Jiangxi Province(20232ACF01001)and the Key Natural Science Foundation of Jiangxi Province(20224ACB205001) （20232ACF01001）
