江西农业大学学报2025，Vol.47Issue(4)：900-908,9.DOI:10.3724/aauj.2025077
烟草苗期耐冷性状关键指标的混合遗传分析
Genetic effect analysis of key indexes of tobacco cold tolerance
摘要
Abstract
[Objective]Tobacco is an important economic crop,and its growth and quality are significantly affected by low temperature.In order to analyze the genetic mechanism of tobacco cold tolerance traits,this study aims to clarify the genetic rules of key indicators of cold tolerance by constructing genetic population and mixed genetic model analysis,thus providing theoretical basis for cold tolerance breeding.[Method]In this study,F1 and F2 genetic populations were constructed from the parents of cold-sensitive variety H382 and cold-tolerant variety QX208,which had significant differences in cold tolerance.After low temperature treatment at seedling stage,the yellowing,softening and new leaves were observed and recorded.The main gene+multi-gene mixed genetic model was used,and the best genetic model was screened by the Chichi Information Criterion(AIC)to estimate the genetic parameters and heritability.[Result]The study found that there were differences in the heritability of genes controlling different traits of tobacco cold tolerance.Leaf yellowing,softening and newborn leaves were controlled by 2 pairs of additive-dominant major genes+additive-dominant polygenes,2 pairs of additive-dominant-epistatic major genes+additive-dominant-epistatic polygenes,and 2 pairs of isomorphic major genes+additive-dominant polygenes,respectively.Among them,the heritability rate of major genes in leaves with yellowing phenotype in F2 population was 58.84%,while the heritability rates of major genes in leaves with softening and newborn leaves were 78.06%and 86.38%,respectively,indicating the importance of major genes in cold tolerance traits.[Conclusion]Tobacco cold tolerance traits are regulated by both main genes and polygenes,the heritability of main genes is significant,and dominant effects have a key impact on phenotype formation.The findings provide a new perspective for the genetic analysis of tobacco cold tolerance traits and offer important genetic parameters for molecular-assisted breeding of cold-tolerant varieties.关键词
烟草耐冷性/主基因加多基因/遗传机制Key words
tobacco cold tolerance/major gene plus polygene/genetic mechanism分类
农业科技引用本文复制引用
马佳洁,张城蓓,籍燕,李媛,杜帅斌,王俊,张兴伟,李丽华,吴则东..烟草苗期耐冷性状关键指标的混合遗传分析[J].江西农业大学学报,2025,47(4):900-908,9.基金项目
中国农业科学院科技创新工程项目(ASTIP-TRIC01)和山东省自然科学基金面上项目(ZR2022MC145) Project supported by the Chinese Academy of Agricultural Sciences Science and Technology Innovation Project(ASTIP-TRIC01)and Shandong Natural Science Foundation Surface Project(ZR2022MC145) 河南省烟草公司洛阳市公司科技项目(2024410300270128),中国烟草总公司四川省公司青年科技托举人才项目(YCQTSC202402),中国烟草总公司四川省公司科技项目(SCYC202302,SCYC202421,SCYC202508)同时对本研究给予了资助,谨致谢意! （ASTIP-TRIC01）
