江西农业大学学报2025，Vol.47Issue(4)：932-946,15.DOI:10.3724/aauj.2025080
枳和甜橙中AUX/LAX和PIN基因家族全基因组鉴定及在缺硼与生长素处理下的表达分析
Genome-wide identification,bioinformatics analysis and expression analysis under boron deficiency and auxin application conditions of AUX/LAX and PIN gene families in citrus
摘要
Abstract
[Objective]Auxin resistant 1/like AUX1(AUX/LAX)and Pin-formed(PIN)are the main carrier proteins for the polar transport of auxin.The aim of this study is to investigate the number of AUX/LAX and PIN gene families in citrus,the physicochemical properties of the predicted proteins and bioinformatics analysis,and their expression patterns analysis under boron deficiency and auxin application conditions.[Method]The genome-wide identification and bioinformatics analysis were conducted in Citrus trifoliata(also known as Poncirus trifoliata)and Citrus sinensis,and their expression patterns under boron deficiency and auxin application conditions were analysed.[Result]The results showed that 4 AUX/LAX family members were identified from the genomic databases of both Citrus trifoliata and Citrus sinensis,while the number of PIN gene family members was 10 and 9,respectively.The physical and chemical properties of predicted proteins showed that the proteins encoded by AUX/LAX gene family in Citrus trifoliata and Citrus sinensis were alkaline unstable hydrophobic proteins,while the proteins encoded by PIN gene were mostly alkaline stable hydrophobic proteins,and the subcellular location of AUX/LAX and PIN proteins was mainly in the plasma membrane.Phylogenetic analysis showed that AUX/LAX gene families were divided into 2 subgroups and PIN was divided into 3 subgroups,with similar gene structure among members of the same subfamily.Analysis of gene conserved motifs showed that PIN gene family conserved motifs were better than AUX/LAX in both location and frequency of occurrence in both Citrus trifoliata and Citrus sinensis.Cis-acting element analysis showed that AUX/LAX and PIN gene families contained elements related to hormone response,abiotic and biological stress,growth and development,secondary metabolism,and photosynthesis in both Citrus trifoliata and Citrus sinensis.Under boron deficiency conditions,there was no significant change in the expression of AUX/LAX gene family gens in the root of trifoliate orange at all treatment periods,but CsLAX2 and CsLAX3 genes of red tangerine were significantly down-regulated at 40 and 80 days of treatment,respectively;The expression of CitPIN5b、CitPIN5c、CitPIN5d and CitPIN8 genes in the root of trifoliate orange were significantly up-regulated in early stages(10 and 20 days),but CitPIN2、CitPIN5b、CitPIN5d genes were significantly down-regulated at 40 days of treatment.However,in the root of red tangerine,only CsPIN1a expression was significantly down-regulated after 10 days,while CsPIN3 and CsPIN5c expression were significantly down-regulated after 40 days of treatment.Under auxin application conditions,there was no significant change in the expression of AUX/LAX gene family gens in the root of trifoliate orange at all treatment periods,but the expression of CsLAX2 gene was significantly down-regulated after 40 days,and CsLAX3 were significantly down-regulated at all treatment periods in the root of red tangerine;The expression of CitPIN2、CitPIN5c and CitPIN5d genes in the root of trifoliate orange were significantly up-regulated in later stages(40 and 80 days),while the expression of CsPIN2 and CsPIN3 genes in the root of red tangerine were significantly down-regulated in later stages,only CsPIN3 genes was significantly up-regulated after 10 days of treatment.[Conclusion]AUX/LAX and PIN gene family members are similar in quantity,structure and predictive protein properties,but their expression levels are significantly different in response to boron deficiency and auxin application treatment.This study provides a basis for further exploring the molecular mechanisms of AUX/LAX and PIN gene families in response to stress and regulation of growth and development in citrus.关键词
柑橘/生物信息学/生长素/植物营养/基因表达Key words
citrus/bioinformatics/auxin/plant nutrition/gene expression分类
农业科技引用本文复制引用
杨梅,李延红,付艺萍,魏清江,周高峰..枳和甜橙中AUX/LAX和PIN基因家族全基因组鉴定及在缺硼与生长素处理下的表达分析[J].江西农业大学学报,2025,47(4):932-946,15.基金项目
国家自然科学基金项目(32160680、31960573) Project supported by the National Natural Science Foundation of China(32160680,31960573) （32160680、31960573）
