解放军医学院学报2025，Vol.46Issue(2)：186-190,204,6.DOI:10.12435/j.issn.2095-5227.23121803
丙戊酸钠对严重烫伤大鼠早期生存率和心肌损伤的作用及机制
Effects and mechanisms of sodium valproate on early survival and myocardial injury in rats with severe scald injury
摘要
Abstract
Background Sodium valproate(VPA)has made great progress in the study of cardiac function protection in the early stage of severe scald injury,hypoxia-inducible factor-1α(HIF-1α)is closely related to myocardial injury,and the mechanism of action of VPA and HIF-1α in myocardial injury in severe scald injury remains to be explored.Objective To investigate whether VPA attenuates early myocardial injury in severely scalded rats by affecting HIF-1α expression.Methods Eighty-eight male SD rats were randomly divided into normal+0.9%NaCl group(NN group,n=24),scald+0.9%NaCl group(SN group,n=32),and scald+VPA group(SV group,n=32);III ° scald with 50%of total surface area were caused by boiling water method,and NN and SN group were intraperitoneally injected with 0.25 mL of NaCl,and the SV group was intraperitoneally given VPA(300 mg/kg,dissolved in 0.25 mL of 0.9%NaCl)immediately after the injury.Eight rats in each group were executed at 3h and 6h after injury,and creatine kinase isoenzyme(CK-MB)levels were measured by taking blood from the abdominal aorta;myocardial tissues were taken for observation of myocardial tissue pathology and apoptotic cardiomyocytes,the levels of tumor necrosis factor-alpha(TNF-α),the expression levels of HIF-1α,BCL2/adenovirus E1B interacting protein 3(BNIP3),and cleaved caspase-3;the 12-hour survival rate was observed in the remaining rats in each group.Results Survival analysis showed that at 12 h after injury,SV group had a mean survival time of(550.00±17.32)min and a survival rate of 50%,which were superior to that of SN group(mean survival time of[400.00±29.76]min and a survival rate of 0%,P＜0.05).Compared with NN group,CK-MB,cardiomyocyte apoptosis rate,TNF-α,cleaved caspase-3,HIF-1α,and BNIP3 protein expression levels increased in SN group at 3h and 6h post-injury(all P＜0.05);compared with SN group,CK-MB,cardiomyocyte apoptosis rate,TNF-α were decreased,and cleaved caspase-3,HIF-1α,and BNIP3 protein expression levels were decreased in SV group(all P＜0.05);pathologic injuries were improved in SV group compared to SN group.Conclusion VPA improves early post-injury survival and attenuates myocardial injury in severely scalded rats,which may be related to the down-regulation of HIF-1α expression in rat myocardial tissue.关键词
烫伤/丙戊酸钠/心肌损伤/凋亡/缺氧诱导因子-1αKey words
scald/sodium valproate/myocardial injury/apoptosis/hypoxia-inducible factor-1α分类
医药卫生引用本文复制引用
孟祥熙,刘翔宇,吴育寿,温海玲,胡森,郑金光..丙戊酸钠对严重烫伤大鼠早期生存率和心肌损伤的作用及机制[J].解放军医学院学报,2025,46(2):186-190,204,6.基金项目
承德市科学技术研究与发展计划项目(201904A106) （201904A106）
