昆明医科大学学报2025，Vol.46Issue(7)：18-25,8.DOI:10.12259/j.issn.2095-610X.S20250703
半枝莲水提物通过调节免疫微环境抑制乳腺癌肺转移
Scutellaria barbata D.Don Water Extract Inhibits Breast Cancer Lung Metastasis by Modulating the Immune Microenvironment
摘要
Abstract
Objective To investigate the mechanism by which Scutellaria barbata D.Don water extract(SBW)inhibits lung metastasis of breast cancer by regulating the immune microenvironment.Methods A mouse model of lung metastasis was established using 4T1 breast cancer cells.Mice were divided into a control group(n=6)and an SBW treatment group(n=6).The effect of SBW on tumor growth was assessed by measuring the volume of the primary tumor,and the inhibitory effect on lung metastasis was evaluated by observing the number and area of metastatic nodules in lung tissue using H&E staining.Flow cytometry was used to analyze changes in the composition of immune cells in the tumor,peripheral blood,and lung tissue.Results Compared with the control group,the SBW treatment group inhibited the growth of the primary tumor(P<0.01)and reduced the number and area of lung metastatic nodules(P<0.01).Flow cytometry analysis showed that after SBW treatment,the numbers of CD86+macrophages(P<0.001)and polymorphonuclear myeloid-derived suppressor cells(PMN-MDSCs)(P<0.05)in the tumor tissue were increased.In lung tissue,the numbers of CD86+macrophages,natural killer T(NKT)cells,natural killer(NK)cells,and PMN-MDSCs were also elevated(P<0.05).Meanwhile,the numbers of regulatory T cells(Tregs)(P<0.05),CD206+macrophages(P<0.01),and monocytic myeloid-derived suppressor cells(M-MDSCs)(P<0.05)in both tumor and lung tissues were decreased.Conclusion SBW inhibits the breast cancer growth and lung metastasis by regulating the recruitment and distribution of immune cells in the tumor,peripheral blood,and lung tissue,thereby enhancing anti-tumor immune responses and reducing immune suppression.关键词
半枝莲/乳腺癌/肺转移/免疫微环境Key words
Scutellaria barbata D.Don/Breast cancer/Lung metastasis/Immune microenvironment分类
医药卫生引用本文复制引用
廖泉炀,任安吕,左晨蓉,李菊,孙瑞芬,苏晓三..半枝莲水提物通过调节免疫微环境抑制乳腺癌肺转移[J].昆明医科大学学报,2025,46(7):18-25,8.基金项目
国家自然科学基金地区科学基金(82060519) （82060519）
云南省中医药基础研究联合专项基金(202301AZ070001-062) （202301AZ070001-062）
云南省应用基础研究中医联合专项基金(2019FF002(-011)) （2019FF002(-011）
云南省教育厅科学研究基金(2024Y355) （2024Y355）
