昆明医科大学学报2025，Vol.46Issue(7)：46-53,8.DOI:10.12259/j.issn.2095-610X.S20250706
EIF5A1通过Wnt/β-Catenin信号通路促进肝内胆管癌细胞增殖和迁移侵袭
EIF5A1 Promotes Proliferation,Migration and Invasion of Intrahepatic Cholangiocarcinoma Cells Through Wnt Signaling Pathway
摘要
Abstract
Objective To investigate the expression of EIF5A1 in intrahepatic cholangiocarcinoma cell lines and human hepatobiliary duct epithelia,and its effect on the proliferation,migration and invasion ability and Wnt/β-Catenin signaling pathway in HUCCT1 cells.Methods Western blot was used to detect the basal expression level of EIF5A1 in intrahepatic cholangiocarcinoma cell lines and human intrahepatic cholangiocarcinoma epithelial cells.Transient transfection of siRNA was used to silence the expression of EIF5A1 in intrahepatic cholangiocarcinoma cell HUCCT1.The experimental groups were divided into blank control group(Con),siRNA1 group,and siRNA2 group.The most effective siRNA was screened by Western blot.The effects of EIF5A1 silencing on the proliferation,migration and invasion ability of HUCCT1 cells were detected by CCK-8,EdU cell proliferation assay and Transwell assay.The effect of EIF5A1 silencing on the Wnt/β-Catenin signaling pathway in HUCCT1 cells was detected by Western blot.Results The results of CCK-8 and EdU cell proliferation experiments showed that the proliferation ability of HUCCT1 cells decreased after EIF5A1 silencing(P<0.05),and Transwell migration and invasion experiments showed that the migration and invasion ability of Hucct1 cells decreased after EIF5A1 silencing(P<0.05).Western blot analysis revealed decreased expression of β-Catenin,Cyclin D1,MMP-2 and Survivin in Wnt/β-Catenin signaling pathway after EIF5A1 silencing(P<0.05).Conclusion EIF5A1 may promote the proliferation,migration and invasion of intrahepatic bile duct cancer cells through Wnt/β-Catenin signaling pathway.关键词
肝内胆管癌/EIF5A1/Wnt/β-Catenin信号通路/增殖/迁移侵袭Key words
Intrahepatic cholangiocarcinoma/EIF5A1/Wnt/β-Catenin signaling pathway/Proliferation/Migration and invasion分类
医药卫生引用本文复制引用
杨少华,许永平,赵棁预,方兴保,阮振兴..EIF5A1通过Wnt/β-Catenin信号通路促进肝内胆管癌细胞增殖和迁移侵袭[J].昆明医科大学学报,2025,46(7):46-53,8.基金项目
云南省教育厅科学研究基金(2024J0320) （2024J0320）
