临床传统医学和药理学（英文）2025，Vol.6Issue(2)：35-43,9.DOI:10.1016/j.ctmp.2025.200204
Androstachys johnsonii Prain叶水提物体外减轻高血糖诱导的精子氧化应激损伤
Androstachys johnsonii Prain aqueous leaf extract attenuates oxidative stress in hyperglycaemic-induced spermatozoa:An in vitro study
摘要
Abstract
Background:Oxidative stress is a key instigator in the development of hyperglycaemia-associated complications in individuals with diabetes mellitus(DM).Male infertility is one of the complications associated with oxidative stress and hyperglycaemia.Androstachys johnsonii Prain has been used traditionally to treat hyperglycaemic-related male infertility.Although its antioxidant capacity and phytochemical composition were previously re-ported,the effect of the plant extract on male parameters under oxidative stress conditions remains unclear. Objective:This study investigated the effect of the aqueous leaf extract of A.johnsonii on hyperglycaemic-induced sperm cells. Methods:A total of 25 male participants,aged 18-40,were recruited voluntarily following ethical guidelines.Sperm parameters,including reactive oxidative species(ROS),total protein content in the plasma,catalase ac-tivity,superoxide dismutase(SOD)activity,DNA fragmentation were measured after hyperglycaemia induction and treatment with(0.001 μg/mL,0.01 μg/mL,0.1 μg/mL,and 1 μg/mL)of plant extract,and 100 μg/mL of acarbose.Capacitation and acrosome reaction,mitochondrial membrane potential,sperm vitality and motility were also assessed. Results:The exposure of sperm cells to hyperglycaemic conditions for 24 h resulted in a significant(P＜0.05)in-crease in the production of reactive oxidative species(ROS),DNA fragmentation,and early capacitation and acro-some reaction,and a significant(P＜0.05)decline in mitochondrial membrane potential(MMP),vitality,motility.After the administration of A.johnsonii extract,there was a significant(P＜0.05)concentration-dependent re-duction in ROS production,DNA fragmentation,and an increase in MMP integrity and vitality.The effect of acarbose on the sperm parameters was comparable to the highest concentrations(0.1 μg/mL and 1 μg/mL)of the extract.However,no significant(P＞0.05)change in motility and vitality was observed after treatment with acar-bose.There was a significant(P＜0.05)reduction of capacitated and acrosome-reacted sperm cells post-acarbose treatment. Conclusion:These findings revealed the evident potential of A.johnsonii in the improvement of male sperm parameters in DM through the reduction of ROS.关键词
Androstachys johnsonii/高血糖/男性不育/氧化应激/精子Key words
Androstachys johnsonii/Hyperglycaemia/Male infertility/Oxidative stress/Spermatozoa引用本文复制引用
Murendeni Nethengwe,Kunle Okaiyeto,Chinyerum S.Opuwari,Oluwafemi O.Oguntibeju..Androstachys johnsonii Prain叶水提物体外减轻高血糖诱导的精子氧化应激损伤[J].临床传统医学和药理学（英文）,2025,6(2):35-43,9.基金项目
This study was funded by Cape Peninsula University of Technology(Reference number:CPUT-RJ-23). （Reference number:CPUT-RJ-23）
