临床传统医学和药理学（英文）2025，Vol.6Issue(2)：66-75,10.DOI:10.1016/j.ctmp.2025.200214
整合代谢组学和网络医学探讨静灵口服液治疗注意缺陷多动障碍的作用机制
Integrated metabolomic analysis and network medicine to investigate the mechanisms of Jingling oral liquid in treating ADHD
摘要
Abstract
Background:Attention deficit hyperactivity disorder(ADHD)is one of most common disorders in child and adolescent psychiatry.Jingling oral liquid,a traditional Chinese patent medicine,is frequently used in clinical practice for treating ADHD,and its therapeutic efficacy is widely recognized.However,its underlying mechanisms are not fully understood. Objective:This study used network medicine and metabolomics to investigate the mechanisms by which Jingling oral liquid alleviates ADHD. Methods:First,dopamine levels in the brain tissue of Wistar-Kyoto and spontaneously hypertensive rats were measured using high-performance liquid chromatography with a fluorescence detector(HPLC-FLD).Second,the Kunming mice were used to analyze the ingredients that reached the brain.The ingredients and targets of Jingling oral liquid for improving ADHD were analyzed through network medicine,and nontargeted metabolomics was used to screen differentially abundant metabolites in brain tissue and analyze possible metabolic pathways. Results:The medium-and high-dose Jingling oral liquid groups showed significantly increased dopamine levels in rat brain tissue.The combined analysis of network medicine and metabolomics revealed that quercetin,berberine,tenuifolin,phellodendrine and gallic acid in Jingling oral liquid regulate 12 key proteins,including APP,BDNF,DRD1-4,and 5-HTR2C.These compounds modulate four metabolites,namely dopamine,γ-aminobutyric acid(γ-GABA),calcitriol,and N-acylsphingosine,which are involved in four metabolic pathways,butanoate metabolism,sphingolipid metabolism,tyrosine metabolism and steroid biosynthesis,to treat ADHD. Conclusion:Jingling oral liquid exerts it therapeutic effects on ADHD through multicomponents,multitargets and multimetabolic pathways,providing an important research basis for the clinical treatment of ADHD.关键词
注意缺陷多动障碍/静灵口服液/网络医学/代谢组学Key words
Attention deficit hyperactivity disorder/Jingling oral liquid/Network medicine/Metabolomics引用本文复制引用
罗威,崔国祯,王雅溶,张世勋,李应应,钟翔宇,吴志文,刘碧连,曹永军,马国,杨庆..整合代谢组学和网络医学探讨静灵口服液治疗注意缺陷多动障碍的作用机制[J].临床传统医学和药理学（英文）,2025,6(2):66-75,10.基金项目
This work was supported by grants from the PhD Start-up Fund of Zunyi Medical University(F-ZH-001)and the Innovation Team Project by the Department of Education of Guangdong Province(Grant No.2024KCXTD005). （F-ZH-001）
