Abstract
Objective:Study on the relationship between nucleotide binding oligomerization domain like receptor 3(NLRP3)signaling pathway factors and the occurrence of peri-implantitis(PI)in patients undergoing dental implant restora-tion,as well as its risk prediction.Methods:Totally 212 patients with dental implant restoration admitted to our hospital from March 2021 to March 2023 were selected as the research subjects.After 3 months,they were divided into PI group(n=37)and non PI group(n=175)according to whether PI occurred.Peri-implant exudate samples were collected from the two groups of patients,subsequently,the exudate samples were separated and processed.Western blot technology was used to de-termine the protein expression levels of NLRP3,cysteine aspartic acid specific protease 1(Caspase-1),and interleukin-1β(IL-1β)in these cells.The general information and protein expression levels of NLRP3,Caspase-1,and IL-1β were compared between the two groups.Using binary logistic regression analysis to identify the factors affecting the occurrence of PI and con-structing a predictive model,and using ROC curve analysis to assess the diagnostic efficacy of NLRP3,Caspase-1,and IL-1β protein expression levels for PI.Results:There were no significant differences between the two groups in age,gender,BMI,smoking history,drinking history,hypertension history,diabetes history,oral disease course,number of implant dentures,im-plant sites,implant reasons,and prosthetic materials(P>0.05).The protein levels of NLRP3,Caspase-1,and IL-1β in the PI group were higher than those in the non PI group,and the differences were statistically significant(P<0.05).NLRP3,Caspase-1,and IL-1β were independent risk factors affecting the occurrence of PI(P<0.05).The AUC values of NLRP3,Caspase-1,IL-1β and the combined prediction were 0.899,0.833,0.812,and 0.943,with sensitivities of 0.865,0.865,0.703,and 0.943,and specificities of 0.846,0.680,0.749,and 0.911,respectively.Delong method test showed that the combined AUC was higher than that of individual indicators,and both sensitivity and specificity were optimal(P<0.05).Conclusion:NLRP3,Caspase-1,and IL-1β are all risk factors for distinguishing PI occurrence in patients undergoing dental implant restoration,and have a certain predictive power for PI occurrence.When the three factors are combined for predic-tion,the power is the highest.关键词
种植义齿修复/NLRP3信号通路因子/种植体周围炎/风险预测/诊断效能Key words
Dental implant restoration/NLRP3 signaling pathway factor/Peri-implantitis/Risk prediction/Diagnos-tic efficacy分类
医药卫生
