临床与实验病理学杂志2025，Vol.41Issue(7)：886-891,6.DOI:10.13315/j.cnki.cjcep.2025.07.007
可变剪切调控因子ARL6IP4表达与结肠癌病理特征及临床预后的相关性分析
Correlation analysis of alternative splicing regulator ARL6IP4 expression with the pathological characteristics and clinical prognosis in colon cancer
摘要
Abstract
Purpose To investigate correlation of ADP ribosylation-like factor 6 interacting protein 4(ARL6IP4)expression with the pathological characteristics and clinical prognosis in primary colon cancer.Methods The ARL6IP4 mRNA expression in tumor and adjacent normal tissues of 133 colon cancer patients was analyzed by RT-qPCR,and its relationship with tumor location,pathological TNM stage,and 3-year survival prognosis was assessed.Additionally,ARL6IP4 protein expression was analyzed by immunohistochemistry in 30 cases,of which 16 cases were analyzed by immunofluorescence.Results The colon cancer presented significantly higher mRNA and protein levels of ARL6IP4 than adjacent normal tissues(t=4.221,P=5.200 × 10-5;t=7.421,P=3.537 × 10-8).The relative ex-pression level of ARL6IP4 mRNA in colon cancer was positively correlated with pathological TNM stage,N stage and M stage(P＜0.05),and negatively correlated with 3-year cumulative survival probability(P＜0.01).Additionally,sig-moid colon cancer presented significantly higher ARL6IP4 expression than other colon cancers,and at the cellular lev-el,ARL6IP4 was predominantly expressed in the cell nucleus.Conclusion The ARL6IP4 expression in colon cancer is higher than that in adjacent normal tissues,which is closely related to tumor metastasis and clinical prognosis.关键词
结肠肿瘤/病理特征/预后评价/ARL6IP4/基因表达Key words
colon neoplasms/pathological characteristics/prognosis assessment/ARL6IP4/gene expression分类
医药卫生引用本文复制引用
杨勇,唐金涛,韩正阳,薛燊,张志云,周文波,陈武..可变剪切调控因子ARL6IP4表达与结肠癌病理特征及临床预后的相关性分析[J].临床与实验病理学杂志,2025,41(7):886-891,6.基金项目
湖北省自然科学基金(2022CFB004)、国药东风总医院卓越计划创新团队项目(2022CO2) Natural Science Foundation of Hubei Province(2022CFB004) （2022CFB004）
Excellence Program Innovation Team Project of Sinopharm Dongfeng General Hospital(2022CO2) （2022CO2）
