环境与职业医学2025，Vol.42Issue(5)：571-577,7.DOI:10.11836/JEOM24455
基于热解吸-气相色谱质谱法研究煤工尘肺患者呼出气中特征挥发性有机物
Characteristic volatile organic compounds in exhaled breath of coal workers' pneumoconiosis patients by thermal desorption gas chromatography-mass spectrometry
摘要
Abstract
[Background]Coal workers' pneumoconiosis is a serious occupational disease in China.Exhaled volatile organic compounds(VOCs)can serve as the"breath fingerprint"of internal pathological processes,which provides a theoretical basis for exhaled VOCs to be used as po-tential non-invasive biomarkers for early diagnosis of coal workers' pneumoconiosis. [Objective]To screen out the characteristic VOCs and important characteristic VOCs of exhaled air in patients with coal workers' pneu-moconiosis,and to explore the potential of these VOCs as biomarkers for early non-invasive diagnosis of the disease. [Methods]In this study,27 VOCs in the exhaled breath of 22 patients with stage Ⅰ coal workers' pneumoconiosis,77 workers exposed to dust,and 92 healthy controls were quantitatively detected by thermal desorption gas chromatography-mass spectrometry(TD-GC-MS).Substances with P＜0.05 in univariate analysis and variable importance projection(VIP)＞1 in supervised orthogonal partial least squares discriminant analysis(OPLS-DA)model were selected as the characteristic VOCs for early diagnosis of coal workers' pneumoconiosis.Age was included in the LASSO regression model as a covariate to screen out important characteristic VOCs,and the diagnostic performance was evaluated by receiver operating characteristic(ROC)curve.Spearman correlation was further used to explore the correlation between important characteristic VOCs and clinical lung function indicators. [Results]Through univariate analysis and OPLS-DA modeling,8 VOCs were selected,including 2-methylpentane,3-methylpentane,n-hexane,methylcyclopentane,n-heptane,methylcyclohexane,4-methyl-2-pentanone,and 2-hexanone,in exhaled breath of patients with coal workers' pneumoconiosis.The concentrations of 4 VOCs,including 3-methylpentane,n-hexane,4-methyl-2-pentanone,and 2-hex-anone,showed a decreasing trend with the increase of dust exposure years.By LASSO regression,the important characteristic VOCs of the coal workers' pneumoconiosis group and the dust exposure group were n-hexane,methylcyclohexane and 4-methyl-2-pentanone,and the important characteristic VOCs of the coal workers' pneumoconiosis group and the healthy group were 2-methyl-pentane and 4-methyl-2-pentanone.The ROC analysis showed that the area under the curve(AUC)of n-hexane,methylcyclohexane,and 4-methyl-2-pentanone were 0.969,0.909,and 0.956,respectively,and the AUC of combined diagnosis was 0.988 and its Youden index was 0.961,suggesting that these results can serve as a valuable reference for further research on early diagnosis.The Correlation analysis found that there was a positive correlation between n-hexane and lung function indicators in the important characteristic VOCs,indicating that it could indirectly reflect the obstruction of lung function ventilation,further proving that important characteristic VOCs have the potential to monitor lung function decline. [Conclusion]Three important characteristic VOCs selected in this study have the potential to be used as non-invasive biomarkers for early diagnosis and disease monitoring of coal workers' pneumoconiosis,and are worthy of further study and verification.关键词
煤工尘肺/呼出气/挥发性有机物/生物标志物/早期诊断Key words
coal workers' pneumoconiosis/exhaled air/volatile organic compound/biomarker/early diagnosis分类
医药卫生引用本文复制引用
何雅珍,孟庆玉,吴智君,樊晶光,丁春光,王均云,冯钰贞,彭方达,刘改生,杨帆,张春民,高汭..基于热解吸-气相色谱质谱法研究煤工尘肺患者呼出气中特征挥发性有机物[J].环境与职业医学,2025,42(5):571-577,7.基金项目
国家卫生健康委职业安全卫生研究中心自管项目(2019009) （2019009）
职业健康安徽省重点实验室开放基金项目(2024ZYJKB002) （2024ZYJKB002）
