辣椒杂志2025，Vol.23Issue(2)：10-20,11.
辣椒CabHLH71-Like基因的克隆与表达分析
Cloning and Expression Analysis of the CabHLH71-Like Gene in Capsicum annuum L
摘要
Abstract
The basic helix-loop-helix(bHLH)gene family,a major class of transcription factors,is widely present in plants and plays crucial roles in regulating plant growth and development,signal transduction mechanisms,biosynthetic activities,and responses to abiotic stresses.To investigate the structural characteristics and functions of the CabHLH71-Like gene and elucidate its molecular regulatory mechanism in pepper,this study utilized a pepper inbred line LY0(Capsicum annuum L.)as the experimental material.The full-length coding sequence of CabHLH71-Like was cloned,followed by bioinformatic analysis and investigation of its spatiotemporal expression patterns.The results revealed that the coding sequence of CabHLH71-Like spans 939 base pairs,encoding 342 amino acids.The protein's molecular formula is C1662H2663N481O523S11,with a relative molecular mass of approximately 38,104.00 Da.The protein was identified as hydrophilic and unstable.Subcellular localization and transmembrane domain analysis predicted that the CabHLH71-Like protein is localized in the nucleus and is a non-transmembrane protein.Phylogenetic tree construction and homologous sequence alignment demonstrated close evolutionary relationships with species such as Capsicum chinense,with all compared sequences containing the conserved bHLH_AtFAMA_like domain.Quantitative real-time PCR results indicated that CabHLH71-Like exhibited the highest expression in LY0 leaves,followed by fruits.During fruit development,its expression increased progressively with ripening.This study lays a foundation for further exploration of the biological functions of the bHLH transcription factor family and the molecular regulatory mechanisms of CabHLH71-Like gene in pepper,providing valuable insights,particularly regarding its role in pepper leaves.关键词
辣椒/转录因子/时空表达/生物信息学/基因克隆Key words
Capsicum annuum L/transcription factor/spatiotemporal expression/bioinformatics/gene cloning引用本文复制引用
李佩茹,方娜英,毛莲珍,沈逸雨,刘周斌..辣椒CabHLH71-Like基因的克隆与表达分析[J].辣椒杂志,2025,23(2):10-20,11.基金项目
湖南省自然科学基金(2025JJ40022) （2025JJ40022）
长沙市科技计划项目(kq2506023) （kq2506023）
国家自然科学基金青年项目(32302574) （32302574）
抗病抗逆辣椒良种攻关项目(2022010501) （2022010501）
