林业工程学报2025，Vol.10Issue(4)：69-77,9.DOI:10.13360/j.issn.2096-1359.202501025
辣木籽多糖亚硒酸酯的制备及其抗运动疲劳分析
Preparation of polysaccharide selenite ester from Moringa oleifera seeds and study of its anti-exercise fatigue effects
摘要
Abstract
Selenium polysaccharides were a type of active polysaccharide with potential polysaccharide activity and the ability to supplement selenium and were considered as an emerging selenium supplement.Taking polysaccharides from Moringa oleifera seeds as the research object,M.oleifera seeds polysaccharide selenite ester was prepared using the HNO3-Na2SeO3 method.This method converted inorganic selenium into organic selenium,and had mild reaction conditions,with barium chloride as a catalyst to accelerate the reaction efficiency.Selenium existed in the form of selenides on the polysaccharide backbone.M.oleifera seed polysaccharides were successfully selenized to form selenite ester polysaccharides.The optimal preparation process was nitric acid mass concentration of 0.5％,reaction temperature of 60 ℃,and reaction time of 8.3 h.Using these process parameters,the selenium content in the M.oleifera seeds polysaccharides selenite ester was(3.89±0.08)mg/g.The infrared spectroscopy was used to determine its functional groups,and the X-ray polycrystalline diffraction was used to determine its crystal structure.The results showed that the spectral analysis of M.oleifera seed polysaccharide selenite ester revealed characteristic absorption peaks corresponding to-Se-O and Se-O-H,indicating the successful selenization of M.oleifera seed polysaccharides to prepare M.oleifera seed polysaccharide selenite ester.M.oleifera seed polysaccharides and M.oleifera seed polysaccharide selenite ester were both semi crystalline structure,and selenization did not alter the crystal structure of polysaccharides.The anti-exercise fatigue effect of M.oleifera seed polysaccharide selenite ester was studied through its effects on weight-bearing swimming,fatigue related indicators,inflammation,and neurotransmitters in mice.The results showed that M.oleifera seed polysaccharide selenite ester effectively prolonged the swimming exhaustion time of mice,reduce the levels of lactic acid,inorganic phosphate,and serum ammonia in mice's serum,and decreased the inflammatory markers TNF-α and IL-1β while increasing the content of IL-10.Additionally,it enhanced the levels of neurotransmitters dopamine(DA)and norepinephrine(NE)in the brainstem and hypothalamus while reducing the levels of serotonin(5-HT)and its metabolite 5-hydroxyindoleacetic acid(5-HIAA).The effect of M.oleifera seed polysaccharide selenite ester was better than that of M.oleifera seed polysaccharides.Therefore,M.oleifera seed polysaccharide selenite ester could achieve anti-exercise fatigue effects by altering mouse biochemical indicators,delaying inflammatory reactions,and regulating neurotransmitter substances.M.oleifera seed polysaccharide selenite ester had strong anti-exercise fatigue activity and could be used as a food additive.This experimental study could provide reference for developing new selenium rich foods and improving the added value of processed M.oleifera seeds.关键词
辣木籽多糖/亚硒酸酯/响应面法优化/运动/抗运动疲劳Key words
Moringa oleifera seeds/selenite ester/response surface optimization/exercise/anti-exercise fatigue分类
化学化工引用本文复制引用
张苗苗..辣木籽多糖亚硒酸酯的制备及其抗运动疲劳分析[J].林业工程学报,2025,10(4):69-77,9.基金项目
河南省高等学校重点科研项目(23B890010) （23B890010）
河南省高校人文社会科学研究一般项目(2023-ZZJH-285) （2023-ZZJH-285）
河南省社科联调研课题项目(SKL-2023-716). （SKL-2023-716）
