棉花学报2025，Vol.37Issue(3)：165-174,10.DOI:10.11963/cs20250025
miR394a-FBX6模块调控棉花黄萎病抗性
The miR394a-FBX6 module regulates cotton resistance to Verticillium wilt
摘要
Abstract
[Objective]This research aimed to investigate the function of the ghr-miR394-GhFBX6 module in cotton resistance to Verticillium wilt and to provide candidate genes for cotton disease-resistant breeding.[Methods]The expression patterns of ghr-miR394a and GhFBX6 were analyzed using quantitative real-time polymerase chain reaction(qRT-PCR).Virus-induced gene silencing(VIGS)was employed to transiently silence ghr-miR394a and GhFBX6 individually,and in combination with the transient overexpression of ghr-miR394a in cotton,to investigate their roles in resistance to Verticillium wilt.The regulatory effect of ghr-miR394a on GhFBX6 was analyzed using the 5'-RNA ligase-mediated rapid amplification of cDNA ends(5'RLM-RACE)technique combined with a luciferase(LUC)reporter system.[Results]qRT-PCR analysis revealed that ghr-miR394a was highly expressed in cotton leaves,followed by stems and roots.Compared with the water treatment,inoculation with Verticillium dahliae significantly downregulated the expression level of ghr-miR394a in roots,while significantly upregulated the expression level of GhFBX6.Compared with control plants,transient silencing of ghr-miR394a in cotton resulted in a significantly reduced disease index and rate of diseased plants,and less fungal biomass accumulation in stem segments during recovery culture,indicating enhanced resistance to Verticillium wilt.In contrast,transient overexpression of ghr-miR394a or silencing of GhFBX6 led to decreased resistance to V.dahliae,as evidenced by a significantly higher disease index and rate of diseased plants,increased fungal biomass accumulation in stem segments of ghr-miR394a-overexpressing plants,and more severe browning of vascular bundles in GhFBX6-silenced plants.The results from 5'RLM-RACE and the LUC reporter system demonstrated that ghr-miR394a inhibits the expression of GhFBX6 at the post-transcriptional level.[Conclusion]ghr-miR394a targets and inhibits the expression of GhFBX6.ghr-miR394a and GhFBX6 play negative and positive regulatory roles,respectively,in the cotton response to Verticillium wilt.关键词
miR394/GhFBX6/棉花/黄萎病/抗病性/病毒诱导的基因沉默Key words
miR394/GhFBX6/cotton/Verticillium wilt/defense/virus-induced gene silencing引用本文复制引用
廖婵娟,李丹,赵文军,吴琪,尹秀娟,钟贵买,王智,胡广,翟俊峰..miR394a-FBX6模块调控棉花黄萎病抗性[J].棉花学报,2025,37(3):165-174,10.基金项目
湖南省教育科学"十四五"规划课题(XJK23CZY054,XJK24QZY004) （XJK23CZY054,XJK24QZY004）
湖南省社会科学成果评审委员会课题(XSP25YBC202) （XSP25YBC202）
重庆社科项目管理中心职业教育改革课题(SSPMC22145) （SSPMC22145）
