棉花学报2025，Vol.37Issue(3)：175-184,10.DOI:10.11963/cs20250011
GhALMT10在干旱胁迫下的功能鉴定
Functional identification of GhALMT10 under drought stress
摘要
Abstract
[Objective]This study aimed to explore the biological functions of one of the aluminum-activated malate transporter(ALMT)family genes,GhALMT10,in the drought resistance of cotton,thereby establishing a foundation for a deeper understanding of the mechanisms of drought resistance in cotton.[Methods]The coding sequence of GhALMT10 gene was amplified from Gossypium hirsutum TM-1 by polymerase chain reaction(PCR),followed by bioinformatics analysis.The expression patterns of this gene in various cotton tissues,as well as under drought stress,were assessed using quantitative real-time PCR(qRT-PCR).Additionally,the biological function of this gene in cotton's response to drought stress was preliminarily verified using virus-induced gene silencing(VIGS)technology.[Results]The coding region of GhALMT10 spans 1 401 bp,encoding a protein composed of 466 amino acid residues,which is predicted to be stable and hydrophobic.Phylogenetic analysis indicated that GhALMT10 is closely related to GrALMT10,GaALMT10-like,HsALMT10,and TcALMT10.Results by qRT-PCR indicated that GhALMT10 is expressed in cotton roots,stems,and leaves,with the highest expression level observed in the roots.Compared with the control treatment with clear water,the expression level of GhALMT10 was low at 3 h of drought sress,and then significantly increased at 6 h and 9 h of drought stress treatment,while it significantly decreased at 24 h.Furthermore,the survival rate of GhALMT10-silenced cotton plants was significantly higher under drought stress compared with the negative control plants.The water loss rate of detached leaves was significantly reduced,the chlorophyll content in leaves after drought treatment was significantly increased,and the malondialdehyde content was significantly decreased in GhALMT10-silenced cotton plants.[Conclusion]The drought tolerance of GhALMT10-silenced plants was significantly enhanced,indicating that GhALMT10 gene negatively regulates drought resistance in cotton.关键词
棉花/GhALMT10/干旱胁迫/表达模式分析/病毒诱导的基因沉默/生理生化指标Key words
cotton/GhALMT10/drought stress/expression pattern analysis/virus-induced gene silencing/physiological and biochemical indicators引用本文复制引用
马鑫,雷建峰,黄诗雨,张国帅,郭浩猛,李月,代培红..GhALMT10在干旱胁迫下的功能鉴定[J].棉花学报,2025,37(3):175-184,10.基金项目
新疆维吾尔自治区"天山英才"培养计划(2024SNGGGCC014) （2024SNGGGCC014）
新疆维吾尔自治区自然科学基金(2022D01A184) （2022D01A184）
新疆维吾尔自治区自然科学基金杰出青年科学基金(2023D01E03) （2023D01E03）
新疆农业大学创新团队开放课题(ITB202106) （ITB202106）
新疆维吾尔自治区大学生创新项目(dxscx2024485) （dxscx2024485）
