南京林业大学学报（自然科学版）2025，Vol.49Issue(4)：12-22,11.DOI:10.12302/j.issn.1000-2006.202506004
平安竹缩短节间形成相关转录因子PjGRAS56功能研究
Functional characterization of PjGRAS56,a transcription factor involved in shortened internode formation in Pseudosasa japonica var.tsutsumiana
摘要
Abstract
[Objective]Internode length is a crucial trait for bamboo industrial utilization.Pseudosasa japonica var.tsutsumiana is a stable dwarf mutant of P.japonica,characterized by shortened,flask-shaped internodes,making it an ideal material for studying internode growth in bamboos.Preliminary studies identified the GRAS transcription factor PjGRAS56 as a key candidate gene responsible for the shortened internode formation in P.japonica var.tsutsumianais.This study aims to elucidate the potential role of PjGRAS56 in bamboo internode development.[Method]We employed bioinformatics to analyze the sequence features of PjGRAS56,confocal laser scanning microscopy to observe its subcellular localization,a yeast transcription system to assess its transcriptional activation activity,transgenic techniques in rice and Arabidopsis to analyze phenotypes of PjGRAS56-overexpressing plants,transcriptome sequencing and qRT-PCR to examine gene expression changes,and hand-sectioning to measure internode cell length.[Result]PjGRAS56 localized to the nucleus and cytoplasm,and lacked transcriptional activation activity.It was significantly down-regulated in the division zone of P.japonica var.tsutsumianais internodes.Overexpression of PjGRAS56 in rice resulted in dwarfism,shortened internodes,reduced tillering,shorter panicles and awns,fewer grains,and reduced leaf angle;overexpression of it in Arabidopsis led to smaller leaves and shorter flower stalks.Although the cell length in internodes of PjGRA4S56-overexpressing plants was similar to controls,the cell number was significantly reduced.Transcriptome sequencing and differential gene expression analysis revealed significant down-regulation of cell division-related genes in Pj6RAS56-overexpressing plants.[Conclusion]PjGRAS56 is a putative negative regulator of internode growth in bamboo.关键词
平安竹/节间长度/居间分生组织/分裂区/PjGRAS56Key words
Pseudosasa japonica var.tsutsumiana/internode length/intercalary meristem/division zone/PjGRAS56分类
农业科技引用本文复制引用
刘世婴,蒋嘉雯,高志鹏,魏强..平安竹缩短节间形成相关转录因子PjGRAS56功能研究[J].南京林业大学学报（自然科学版）,2025,49(4):12-22,11.基金项目
国家自然科学基金项目(32071848,32471977). （32071848,32471977）
