南京林业大学学报（自然科学版）2025，Vol.49Issue(4)：46-56,11.DOI:10.12302/j.issn.1000-2006.202412025
转录因子等在植物体胚发生中的作用机制
Mechanisms of transcription factors and others in somatic embryogenesis of plants
摘要
Abstract
Somatic embryogenesis is one of the key ways to achieve asexual reproduction of plants under in vitro culture conditions.The process undergoes somatic cell,treated by the exogenous hormone,reprogramming to form a zygote-like structure that eventually develops into a complete plant.As an efficient plant regeneration technology,somatic embryogenesis has the potential to be applied in plant Agrobacterium-mediated genetic transformation and CRISPR-based genome editing technologies,and is of great value in genetic improvement of plants.Recent advances have revealed that somatic embryogenesis is governed by a hierarchical molecular network involving multiple regulatory tiers.In this review,we review the recent research progress on the molecular regulatory mechanisms of somatic embryogenesis in plants,focusing on the key transcription factors,such as WOX(WUSCHEL-RELATED HOMEOBOX)gene family,AP2/ERF(APETALA2/ETHYLENE-RESPONSIVE FACTOR)and MADS-box gene family,as well as the related signaling pathways in which they are involved in.The members of WOX gene family,particularly WUS and WOX2/5/8/9,play a central role in maintaining stem cell pluripotency and establishing polarity during the development of embryos.The LAFL network(LEC1-ABI3-FUS3-LEC2),integrates hormonal signals,which regulate auxin biosynthesis(YUCs and TAA1)and the balance of endogenous ABA/GA,and epigenetic signals,which modified epigenetically,to regulate somatic embryogenesis.AP2/ERF superfamily members,notably BABY BOOM and PLETHORA5(PLT5),are essential for somatic cells to acquire totipotency.Members of the MADS-box gene family,including AGL15 and AGL18,regulate somatic embryogenesis mainly by modulating GA-related pathways.These transcription factors play crucial roles in somatic embryogenesis.This paper delves into the broad application prospects of somatic embryogenesis in plant breeding and other biotechnology field.With the deepening of research,this technology will occupy a central position in plant biotechnology and agricultural development.关键词
体胚发生/分子调控/转录因子/表观遗传Key words
somatic embryogenesis/molecular regulation/transcription factors/epigenetics分类
农业科技引用本文复制引用
张稼霁,郝兆东,鲁路,郑仁华,施季森,陈金慧..转录因子等在植物体胚发生中的作用机制[J].南京林业大学学报（自然科学版）,2025,49(4):46-56,11.基金项目
国家重点研发计划(2023YFD2200101) （2023YFD2200101）
福建省林业科技项目(2024FKJ05) （2024FKJ05）
江苏省高等学校优势学科建设项目(PAPD). （PAPD）
