南京林业大学学报（自然科学版）2025，Vol.49Issue(4)：71-78,8.DOI:10.12302/j.issn.1000-2006.202409034
外源脱落酸与低温预处理结合对红松体胚成熟的影响
Effect of exogenous abscisic acid combined with low temperature pretreatment on Pinus koraiensis somatic embryo maturation
摘要
Abstract
[Objective]This study aims to elucidate the effects of exogenousabscisic acid(ABA)combined with low-temperature pretreatment on the maturation of Pinus koraiensis somatic embryos,unravel the mechanisms regulating embryogenic cell differentiation and metabolite accumulation,and identify the optimal culture conditions.[Method]Using P.koraiensis embryogenic callus as the material,four cold durations(0,2,4 and 6 days)at 4℃ were combined with six ABA concentrations(0,5,10,20,50,100 mg/L)medium.The activity of nutrients and related physiological and biochemical indicators the callus were measured,and the effects of different treatment combinations on somatic embryo maturation were systematically evaluated.[Result](1)20 mg/L ABA with two days cold pretreatment increased somatic embryo yield 9.90 times versus control.(2)In this treatment group,the contents of storage substances reached the highest levels,and activities of peroxidase(POD)and catalase(CAT)were also at their peak,reaching 59.45 and 98.96 U/g,respectively.Meanwhile,H2O2 content was the lowest at 0.53 μmol/mg,indicating that the antioxidant system effectively mitigated oxidative damage.(3)Somatic embryos obtained under optimized culture conditions exhibited a regeneration rate of 45％after germination and successful transplantation.[Conclusion]The combination of 20 mg/L exogenous ABA with low-temperature pretreatment at 4℃ for two days was identified as the optimal condition for somatic embryo induction and maturation in P.koraiensis.This study provides technical support for the efficient propagation of elite P.koraiensis varieties,contributing to the effective conservation of genetic resources,the selection of superior cultivars,and the development of a rapid propagation system.关键词
红松/体胚成熟/脱落酸/低温预处理/生理生化Key words
Pinus koraiensis/somatic embryo maturation/abscisic acid(ABA)/low temperature pretreatment/physiology and biochemistry分类
农业科技引用本文复制引用
史玉洁,任悦,汤铄然,杨玲..外源脱落酸与低温预处理结合对红松体胚成熟的影响[J].南京林业大学学报（自然科学版）,2025,49(4):71-78,8.基金项目
国家重点研发计划(2023YFD2200103). （2023YFD2200103）
