南京林业大学学报（自然科学版）2025，Vol.49Issue(4)：79-87,9.DOI:10.12302/j.issn.1000-2006.202411019
欧洲小叶椴体细胞胚胎发生及植株再生体系建立
Plant regeneration through somatic embryogenesis in Tilia cordata
摘要
Abstract
[Objective]This study aims to develop a complete somatic embryogenesis system for Tilia cordata by systematically optimizing the culture medium composition to identify optimal conditions for somatic embryo induction and subsequent plant regeneration.[Method]Immature zygotic embryos of T.cordata were used as explants.The effects of different concentrations and combinations of plant growth regulators 2,4-dichlorophenoxyacetic acid(2,4-D and 6-benzylaminopurine(6-BA)on callus induction,proliferation,and somatic embryo induction efficiency were systematically investigated.Additionally,the impacts of different basal media,indole-3-butyric acid(IBA)concentrations,and activated charcoal supplementation on somatic embryo germination and plant regeneration were evaluated.[Result]Optimal callus induction was achieved at a concentration of 3.0 mg/L 2,4-D combined with 0.2 mg/L 6-BA,yielding an induction efficiency of 64.25％.Subsequent proliferation of embryogenic callus demonstrated maximal growth potential under identical hormonal conditions(3.0 mg/L 2,4-D+0.2 mg/L 6-BA),as evidenced by an exceptional proliferation coefficient of 1 063.72％.For somatic embryo induction,the same hormonal formulation(3.0 mg/L 2,4-D+0.2 mg/L 6-BA)proved most effective,generating somatic embryos at a frequency of 46.41％.Complete plant regeneration was successfully accomplished through transfering of developed somatic embryos to MS basal medium supplemented with 0.5 mg/L IBA in the absence of activated charcoal,with all regenerated plants exhibiting normal morphological characteristics.[Conclusion]In this study,a complete somatic embryogenesis system of T.cordata was initially established,providing a basis for subsequent establishment of an efficient propagation and genetic transformation system for Tilia.关键词
欧洲小叶椴/胚性愈伤组织/体细胞胚胎/体胚再生Key words
Tilia cordata/embryogenic callus/somatic embryo/somatic embryo regeneration分类
农业科技引用本文复制引用
李尹,于丝浓,严灵君,王欢利,汤诗杰..欧洲小叶椴体细胞胚胎发生及植株再生体系建立[J].南京林业大学学报（自然科学版）,2025,49(4):79-87,9.基金项目
江苏省农业科技自主创新项目(CX[22]3177) （CX[22]3177）
南京绿化园林局科技项目(YLKJ 202214JH) （YLKJ 202214JH）
江苏省中国科学院植物研究所院士创新高地项目(JSPKLB2023060) （JSPKLB2023060）
中国工程院战略研究与咨询项目(2023-XZ-83) （2023-XZ-83）
国家自然科学基金项目(31700477) （31700477）
江苏省中国科学院植物研究所乡土景观树种种质资源库项目. （）
