南京农业大学学报2025，Vol.48Issue(4)：781-789,9.DOI:10.7685/jnau.202403024
木薯MeTGA9.3转录因子鉴定及其下游调控基因预测
Identification of MeTGA9.3 transcription factor and prediction of its downstream regulating genes in cassava
摘要
Abstract
[Objectives]This paper aimed to identify the target genes regulated by the cassava TGA transcription factor MeTGA9.3,and lay the foundation for further studying the function of this transcription factor in regulating the synergistic and efficient utilization of water and nitrogen nutrients in cassava.[Methods]The transcription factor characteristics of MeTGA9.3 were determined based on subcellular localization and transcriptional activation experiments.DAP-Seq was used to identify the DNA elements recognized and bound by MeTGA9.3 at the whole genome level.[Results]The MeTGA9.3 protein localized in the nucleus and exhibited significant transcriptional activation activity in yeast.The MeTGA9.3 promoter drove the reporter gene GUS significantly expressing in the roots of transgenic Arabidopsis thaliana.The advantageous DNA element for MeTGA9.3 transcription factor recognition and binding was TGACGTCA.In the predicted target genes of MeTGA9.3,several LEA,ERF,and MYC members related to stress were found,as well as three members of nitrogen transport protein 1(NRT1).[Conclusions]This study characterized cassava TGA transcription factor MeTGA9.3,and found that MeTGA9.3 had the potential to synergistically regulate drought stress response and nitrogen transport.关键词
木薯/TGA/转录因子/DAP-Seq/干旱胁迫/氮素转运蛋白Key words
cassava/TGA/transcription factor/DAP-Seq/drought stress/nitrogen transport protein分类
农业科技引用本文复制引用
张宏图,熊华,江李伟,张圆雷,于晓玲,曾长英,阮孟斌..木薯MeTGA9.3转录因子鉴定及其下游调控基因预测[J].南京农业大学学报,2025,48(4):781-789,9.基金项目
国家自然科学基金项目(32272037) （32272037）
中国热带农业科学院中央公益性科研单位基础研究基金(1630052022008) （1630052022008）
海南省自然科学基金项目(322MS146) （322MS146）
