南京农业大学学报2025，Vol.48Issue(4)：812-822,11.DOI:10.7685/jnau.202405023
菊花茎部腐烂类病害病原菌分离与鉴定
Isolation and identification of pathogen lead to stem rot in Chrysanthemum morifolium
摘要
Abstract
[Objectives]The paper aimed to identify the main pathogenic fungi that cause chrysanthemum stem rot disease.[Methods]The chrysanthemum with decaying stem was collected from the chrysanthemum planting base as the research materials,and the tissue at the junction of diseased and healthy areas was collected and the pathogenic fungi were isolated and purified using the tissue separation method.Subsequently,the rDNA-ITS and EF-1α sequences in the pathogenic fungi were cloned through polymerase chain reaction and sequenced,and a phylogenetic tree was constructed based on these sequences to conduct molecular biological identification of these isolated fungi.The types of pathogens were further confirmed by observing the morphology of hyphae and conidia.The pathogenicity and the Koch postulates assays confirmed that the isolated strains were the pathogenic fungi that caused the rot disease of chrysanthemum stem.[Results]A total of 10 diseased plants with rot stems were collected in this study,and 19 strains of pathogenic fungi were isolated and identified,including 9 strains of Fusarium solani,8 strains of Fusarium commune,and 2 strains of Fusarium oxysporum,all of which belonged to Fusarium species.The pathogenicity assays showed that the isolated strains were all pathogenic to chrysanthemum,and the strains showed different levels of pathogenicity to chrysanthemum.Among the pathogenic fungi,FN3-3 was the most pathogenic strain of F.solani,DT3-3 was the most pathogenic strain of F.oxysporum,and HY1-3 was the most virulent of F.commune.[Conclusions]This study clarified the main pathogenic fungal types of chrysanthemum stem rot disease and provided important clues and basis for the formulation of effective control methods for stem rot disease,the screening of stem rot resistant varieties and disease-resistant breeding.关键词
菊花/茎部腐烂/镰刀菌属/病原菌Key words
Chrysanthemum morifolium/stem rot/Fusarium/pathogen分类
农业科技引用本文复制引用
罗梦熙,姜奕丰,何宇凡,房伟民,陈发棣,管志勇,刘晔..菊花茎部腐烂类病害病原菌分离与鉴定[J].南京农业大学学报,2025,48(4):812-822,11.基金项目
国家重点研发计划项目(2019YFD1001500) （2019YFD1001500）
国家自然科学基金项目(32371949) （32371949）
