南京中医药大学学报2025，Vol.41Issue(7)：869-880,12.DOI:10.14148/j.issn.1672-0482.2025.0869
基于GES-1细胞模型和鼠胃类器官模型的金红片功效物质基础研究
Study on Functional Substance Basis of Jinhong Tablet Based on GES-1 Cell Model and Mouse Gastric Organoid Model
摘要
Abstract
OBJECTIVE To explore the functional substance basis of Jinhong Tablet in the treatment of chronic superficial gastri-tis(CSG).METHODS Three different models were constructed to investigate the anti-inflammatory and antioxidant effects,func-tional material basis of Jinhong Tablet:inflammatory model in human gastric epithelial cells(GES-1)induced by lipopolysaccharide(LPS),LPS-induced inflammatory model in mouse gastric organoids,and ethanol-induced oxidative damage model in GES-1 cells.MTS assay was performed to detect cell proliferation activity;qPCR was applied to measure the relative mRNA expression of tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),interleukin-6(IL-6),and interleukin-8(IL-8)in cells and gastric organoids;and the levels of superoxide dismutase(SOD),malondialdehyde(MDA),and reactive oxygen species(ROS)in cells were detected.RESULTS Jinhong Tablet and 10 functional components significantly reduced the relative expression of inflammation-relat-ed genes TNF-α,IL-1β,IL-6,and IL-8 in LPS-induced GES-1 cells and gastric organoids,suggesting that these 10 components are the functional substance basis for the anti-inflammatory effects of Jin Hong Tablet.Jin Hong Tablet and 11 functional components markedly decreased the levels of MDA and ROS and increased the activity of SOD,indicating that these 11 components were the func-tional substance basis of the antioxidant effects of Jinhong Tablet.CONCLUSION Through in vitro cell and gastric organoid experi-ments,it has been preliminarily determined that allocryptopine,corydaline,dehydrocorydaline,palmatine hydrochloride,chlorogenic acid,costunolide,rutin,quercitrin,dehydrocostus lactone,tetrahydrocoptisine,isochlorogenic acid B,toosendanin,protopine,and quercetin are the functional material basis of Jinhong Tablet in treating CSG,accumulating scientific evidence for the enhancement of the quality standards of Jinhong Tablet.关键词
金红片/慢性浅表性胃炎/功效物质基础/抗炎/抗氧化Key words
Jinhong Tablet/chronic superficial gastritis/functional substance basis/anti-inflammatory/antioxidation分类
医药卫生引用本文复制引用
肖立皓,赵文静,朱高双,闫雨娇,张新庄,曹亮,王振中,范小雪,张彤..基于GES-1细胞模型和鼠胃类器官模型的金红片功效物质基础研究[J].南京中医药大学学报,2025,41(7):869-880,12.基金项目
江苏省基础研究计划自然科学基金——前沿引领技术基础研究专项(BK20232014) （BK20232014）
