南京中医药大学学报2025，Vol.41Issue(7)：896-903,8.DOI:10.14148/j.issn.1672-0482.2025.0896
基于UPLC-Q-TOF/MS分析炙甘草汤体内成分及心脏组织分布特征
Identification of in vivo Components and Distribution Characteristics in Rats,Cardiac Tissues of Zhigancao Decoction by UP-LC-Q-TOF/MS
摘要
Abstract
OBJECTIVE To deeply explore the in vivo pharmacodynamic substance basis of Zhigancao Decoction,a classic tradi-tional Chinese medicine formula,and provide scientific evidence for its rational application and development in modern clinical practice.METHODS Wistar rats were treated with 12.15 g·kg-1 Zhigancao Decoction by gavage.Rat plasma samples were collect-ed at 10 time points(5,15,30,60,120,240,360,480,600 and 720 min after administration)and rat heart(atrial and ventricu-lar)tissue samples were collected at 12 h after administration.Components in the plasma and heart samples were qualitatively identi-fied by ultra-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry(UPLC-Q-TOF/MS),and the distri-bution characteristics of Zhigancao Decoction in vivo were analyzed.At the same time,the time-concentration curve of the prototype components and metabolites in Zhigancao Decoction was drawn to observe the changes of blood drug concentration.RESULTS A total of 11 prototype components(Ajugol,Nicotiflorin,Isoschaftoside,4-Hydroxycinnamic acid,Rehmapicrogenin,4-Hydroxybenzoic acid,4′,7-Dihydroxyflavone,Calycosin,3′,4′,7-Trihydroxyflavone,Pinellic acid,Truxillic acid)and 7 metabolites were identified from the plasma samples of Zhigancao Decoction,mainly including flavonoids(flavonoids glycosides),organic acids,and iridoid glyco-sides,etc.Additionally,6 prototype components(Ajugol,Isoschaftoside,Rehmapicrogenin,4′,7-Dihydroxyflavone,Liquiritigenin,3′,4′,7-Trihydroxyflavone)and 3 metabolites were identified from the cardiac samples(the atrium and the ventricle showed the same results).The metabolic pathways mainly involved Phase Ⅰ metabolism and glucuronidation.CONCLUSION The prototype compo-nents and metabolites in plasma and heart tissue of Zhigancao Decoction is preliminarily determined,providing a reference for analyzing the active components of Zhigancao Decoction in heart tissue.关键词
UPLC-Q-TOF/MS/炙甘草汤/入血成分/代谢产物/心脏Key words
UPLC-Q-TOF/MS/Zhigancao Decoction/blood-absorbed components/metabolites/heart分类
医药卫生引用本文复制引用
唐志苓,李瑶,支颢,王敬彦,蔡雪婷,曹鹏,周谦..基于UPLC-Q-TOF/MS分析炙甘草汤体内成分及心脏组织分布特征[J].南京中医药大学学报,2025,41(7):896-903,8.基金项目
国家重点研发计划项目(2023YFC2308200) （2023YFC2308200）
江苏省中医药科技发展计划项目(ZT202109) （ZT202109）
