农业生物技术学报2025，Vol.33Issue(8)：1645-1660,16.DOI:10.3969/j.issn.1674-7968.2025.08.001
大豆GmSDIR1基因的克隆及功能分析
Cloning and Functional Analysis of GmSDIR1 Gene in Soybean(Glycine max)
摘要
Abstract
Salt-and drought-induced ring finger protein(SDIR)plays an important role in abscisic acid(ABA)-mediated plant stress response,and SDIR is rarely reported in soybean(Glycine max).In this study,the candidate gene GmSDIR1 was screened and cloned from soybean by co-expression analysis of transcriptome sequencing data and weighted gene co-expression network analysis(WGCNA).It was located on chromosome 11,with a full-length coding region of 825 bp,belonging to C3H2C3 type RING finger protein.Subcellular localization showed that GmSDIR1 protein was localized in the nucleus.The expression pattern analysis showed that GmSDIR1 gene was expressed in true leaf,cotyledon and stem tissues of soybean and had spatial and temporal expression specificity.High salt(NaCl),drought(PEG6000),abscisic acid(ABA)and low temperature(4℃)stress treatments could significantly induce the expression of GmSDIR1 gene.Phenotypic analysis showed that tobacco(Nicotiana tobacum)overexpressing GmSDIR1 gene was tolerant to high salt stress,and the heterologous expression of GmSDIR1 promoted seed germination,root length elongation and fresh weight increase of seedlings,and improved the antioxidant capacity of transgenic tobacco.The results of qRT-PCR showed that the expression of GmSDIR1 in transgenic tobacco was significantly activated under high salt stress,which indicated that GmSDIR1 might be involved in the regulation of plant response to salt stress.This study provides an important experimental basis for further analysis of the molecular mechanism of soybean GmSDIR1 gene regulating plant high salt stress response.关键词
大豆/盐和干旱诱导的环指蛋白(SDIR)/基因克隆/高盐/功能Key words
Soybean/Salt-and drought-induced ring finger protein(SDIR)/Gene cloning/High salinity/Function分类
农业科技引用本文复制引用
朱贵爽,赵颖,王晓东,张巍巍,向殿军,李志刚..大豆GmSDIR1基因的克隆及功能分析[J].农业生物技术学报,2025,33(8):1645-1660,16.基金项目
内蒙古自治区一流种业振兴创新团队建设项目(GXKY25Z049) （GXKY25Z049）
内蒙古民族大学与吉林省农科院校地合作项目-横向课题(S23011) （S23011）
内蒙古自治区自然科学基金(2022MS03063) （2022MS03063）
