农业生物技术学报2025，Vol.33Issue(8)：1661-1673,13.DOI:10.3969/j.issn.1674-7968.2025.08.002
独脚金内酯对棉纤维发育的影响及D27基因的表达特性分析
Effect of Strigolactones on Cotton Fiber Development and Analysis of Expression Characteristics of D27 Gene
摘要
Abstract
As a plant hormone,strigolactones has a regulatory effect on cotton fiber development.This study utilized cotton(Gossypium)ovule culture technology to optimize the ovule culture system.15 μmol/L strigolactone(GR24)and 0.1,1,and 5 μmol/L Tis108 were added to treat cotton ovules to screen for Tis108 concentrations that inhibit fiber elongation and explore the effects of strigolactone and its inhibitors on ovule quality and fiber length;Using qRT-PCR to analyze the expression of the β-carotene isomerase D27 gene(D27)in cotton fibers on 5,10,15,20,25,and 30 d,which was involved in the synthesis pathway of lactone.The results showed that:1)In 30 d cotton fibers,compared with the control group,treatment with 15 μmol/L GR24 increased cotton fiber length by 2.07 mm,and treatment with 5 μmol/L Tis108 decreased cotton fiber length by 2.22 mm;2)Compared with the control group,the 6 Gossypium hirsutum D27 gene members showed higher expression levels and differential expression in fibers treated with GR24 on 5 d at different stages;3)Fibers from 6 periods(5,10,15,20,25,and 30 d)in the island cotton field environment,6 GbD27 genes showed different expression characteristics among the fibers.The expression of GbD27-4 gene was more significant in the fibers on 5 and 10 d.This study preliminarily explored the in vitro culture conditions of cotton ovules,screened suitable concentrations of strigolactones inhibitors,and found that the D27 gene was significantly expressed in the early stage of fiber development,provides a theoretical basis for further research on the molecular biology function of cotton D27 gene.关键词
棉纤维/β-胡萝卜素异构酶/胚珠培养/表达分析/独脚金内酯Key words
Cotton fiber/β-carotene isomerase/Ovule culture/Expression analysis/Strigolactone分类
农业科技引用本文复制引用
周强,李颖,孙国清,陈琴,蔡永生,陈全家,曲延英,郑凯..独脚金内酯对棉纤维发育的影响及D27基因的表达特性分析[J].农业生物技术学报,2025,33(8):1661-1673,13.基金项目
新疆维吾尔自治区重点研发项目(2024A0003-2) （2024A0003-2）
"天山英才"培养计划项目(2024TSYCCX0031) （2024TSYCCX0031）
新疆长绒棉育种联合攻关项目(2022MH-01) （2022MH-01）
