农业生物技术学报2025，Vol.33Issue(8)：1674-1682,9.DOI:10.3969/j.issn.1674-7968.2025.08.003
月季RhMYB23基因的克隆及功能分析
Cloning and Functional Analysis of RhMYB23 in Rosa hybrida
摘要
Abstract
The MYB transcription factors constitute one of the largest families of transcription factors in plants and are extensively involved in plant growth and development as well as the biosynthesis of various secondary metabolites.To explore the regulatory function of MYB transcription factors in the biosynthesis of proanthocyanidins in Rosa hybrida,this study used R.hybrida'Violet'as the experimental material and isolated RhMYB23(GenBank No.PQ441876)from its petals.The ORF of RhMYB23 was 771 bp and encoded 256 amino acids.Multiple sequence alignment and phylogenetic analysis revealed that RhMYB23 had a conserved R2R3 domain and was most closely related to Arabidopsis thaliana AtTT2(transparent testa 2).Expression pattern analysis revealed that RhMYB23 was predominantly expressed in R.hybrida petals.RhMYB23 was localized in the nucleus and possessed transcriptional activation activity.Transient overexpression of RhMYB23 in R.hybrida petals significantly increased the accumulation of proanthocyanidins(P＜0.05)and significantly upregulated the expression of key genes involved in proanthocyanidin biosynthesis,RhANR(anthocyanin reductase)and RhLAR(leucoanthocyanidin reductase)(P＜0.001).These results suggested that RhMYB23 in R.hybrida might positively regulate proanthocyanidin synthesis.This study reveals the crucial regulatory role of RhMYB23 in proanthocyanidin biosynthesis in R.hybrida,offering theoretical support for R.hybrida flower color improvement.关键词
月季/RhMYB23/花色/原花青素Key words
Rosa hybrida/RhMYB23/Flower color/Proanthocyanidin分类
农业科技引用本文复制引用
付庭睿,张环宇,崔永一,罗平..月季RhMYB23基因的克隆及功能分析[J].农业生物技术学报,2025,33(8):1674-1682,9.基金项目
浙江省自然科学基金(LZ23C160003) （LZ23C160003）
