农业生物技术学报2025，Vol.33Issue(8)：1732-1742,11.DOI:10.3969/j.issn.1674-7968.2025.08.007
铁皮石斛转醛醇酶DoTAL1基因的克隆及其功能分析
Cloning and Function Analysis of DoTAL1 Gene in Dendrobium officinale
摘要
Abstract
Transaldolase(TAL)plays an important role in plant growth.In order to investigate the growth relationship between TAL gene and D.officinale,in this study screened a significantly differentially expressed gene DoTAL1 was screened from the transcriptome database of D.officinale with growth-promoting effects that inoculated with Epulorhiza in the early stage.The DoTAL1 gene was cloned and functionally studied.The results showed that the full-length cDNA sequence of the DoTAL1 gene was 1 323 bp,encoding 440 amino acids,and it was classified as a member of the triosephosphate isomerase superfamily.Phylogenetic analysis showed that the DoTAL1 protein had the closest evolutionary relationship with the homologous TAL protein of D.nobile.Subcellular localization studies showed that DoTAL1 was located in the cell membrane and chloroplasts.The height of the N.benthamiana overexpressing DoTAL1 was significantly higher than that of the control.After DoTAL1 was silenced,the growth of the Nicotiana benthamiana was slowed down.DoTAL1 was expressed in all tissues of D.officinale,with the highest expression during the vigorous growth period of stem.Its expression was upregulated after establishing symbiosis with Epulorhiza sp.in roots.The expression level of DoTAL1 was positively correlated with plant growth,indicating that DoTAL1 played a critical role in the growth and development of D.officinale.This study provides a theoretical basis for elucidating the metabolic mechanism of DoTAL1 and for the application of mycorrhizal fungi in the cultivation and molecular breeding of orchid plants.关键词
铁皮石斛/转醛醇酶/亚细胞定位/过表达/基因沉默Key words
Dendrobium officinale/Transaldolase(TAL)/Subcellular localization/Overexpression/Gene silencing分类
农业科技引用本文复制引用
王伟英,林文忠,邹晖,林江波,戴艺民..铁皮石斛转醛醇酶DoTAL1基因的克隆及其功能分析[J].农业生物技术学报,2025,33(8):1732-1742,11.基金项目
福建省公益类竞争性项目(2023R1093) （2023R1093）
福建省自然科学基金(2024J011368) （2024J011368）
