农业生物技术学报2025，Vol.33Issue(8)：1743-1754,12.DOI:10.3969/j.issn.1674-7968.2025.08.008
山奈酚促进林下经济植物根系生长与抗旱机制分析
Analysis on the Promotion of Root Growth and Drought Resistance Mechanism of Understory Economic Plants by Kaempferol
摘要
Abstract
Elucidating the regulatory mechanisms of plant secondary metabolites and their associated genes under drought stress is fundamental for enhancing crop resistance and improving yield.Kaempferol is a flavonoid compound widely present in plants,and the mechanisms by which it promotes plant growth and development as well as induces plant stress resistance remain to be studied.This study conducted differential metabolite analysis on the rhizosphere metabolites of Scutellaria baicalensis under monoculture and intercropping conditions,and performed transcriptome sequencing on S.baicalensis samples,the results showed that the growth performance of S.baicalensis under intercropping was significantly higher than that under monocropping.Metabolomic profiling identified significantly elevated levels of kaempferol as a key differential secondary metabolite.Transcriptomic analysis further pinpointed the microtubule-associated protein WVD2-like 4 as markedly upregulated.Tissue-specific expression analysis revealed that WVD2-like 4 was highly expressed in the stems and roots of the understory Cajanus cajan.Subsequent studies revealed that WVD2-like 4 functions as a microtubule-stabilizing protein,and Kaempferol promoted microtubule rearrangement by enhancing WVD2-like 4 transcriptional expression.WVD2-like 4 acted as a positive regulator of root growth,promoting root elongation and enhancing drought tolerance in C.cajan.This research provides novel insights into mining drought-resistant genes from understory plants and unravels the mechanism by which kaempferol regulates microtubule dynamics to enhance drought resistance in understory vegetation.关键词
山奈酚/微管/根系/耐旱性Key words
Kaempferol/Microtubules/Root system/Drought resistance分类
农业科技引用本文复制引用
陈杨,朱甜甜,SUPRUN Ivan Ivanovich,付玉杰,杨清,董碧莹,孟冬..山奈酚促进林下经济植物根系生长与抗旱机制分析[J].农业生物技术学报,2025,33(8):1743-1754,12.基金项目
"十四五"国家重点研发计划(2023YFD2201801) （2023YFD2201801）
国家自然科学基金(32371835 （）
32171742 （）
32401533) （）
国家资助博士后研究人员计划B档(GZB20230059) （GZB20230059）
